alexa Evidence For A Critical Role Of Catecholamines For Cardiomyocyte Lineage Commitment In Murine Embryonic Stem Cells | 22286
ISSN: 2157-7633

Journal of Stem Cell Research & Therapy
Open Access

Like us on:

OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations
700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

3rd International Conference and Exhibition on Cell & Gene Therapy

Martin Lehmann
ScientificTracks Abstracts: J Stem Cell Res Ther
DOI: 10.4172/2157-7633.S1.007
Abstract
Embryonic stem (ES) cells are pluripotent cells derived from the inner cell mass of the blastocyst. These cells possess the ability of infinite self-renewal and to differentiate into all cell types of the three germ-layers. In defined conditions ES cells are committed to the mesodermal lineage and differentiate, amongst other cell types, into cardiomyocytes (CMs). The processes underlying mesodermal and subsequent cardiac differentiation are yet only partially understood. Catecholamine release is well known to modulate heart rate and force in adult mammals. Despite first evidence, only little is known about an involvement of catecholamines during embryonic heart development. Therefore our work aimed to investigate in more detail whether catecholamines are involved in the process of ES cell cardiac differentiation in vitro. Effects of catecholamine depletion induced by reserpine were investigated during murine D3 aPIG44 ES cell differentiation. Reserpine is a drug blocking vesicular storage of monoamines, and as a result depletes cells of the catecholamines norepinephrine and epinephrine. Cardiac differentiation was assessed by quantification of beating clusters, immunocytochemistry, molecular biology, flowcytometry and pharmacological approaches. Proliferation and cytotoxicity was evaluated by embryoid body cross-section measurements and impedance monitoring, while functional characterization of CMs was performed using extracellular field potential (FP) recordings with microelectrode arrays (MEAs). Involvement of b-adrenoceptor signaling was studied differentiating ES cells in the presence of reserpine and isoproterenol. To further discriminate between drug-specific effects of reserpine and catecholamine action via adrenergic receptors we applied the unspecific α- and β-receptor antagonists phentolamine and propranolol during differentiation. Reserpine treatment led to a remarkable reduction of beating cardiac clusters, downregulation of cardiac proteins α-actin and troponinT and delayed mesodermal and cardiac gene expression. In more detail, the average ratio of ~40% spontaneously beating control clusters was significantly reduced by 100%, 91.1% and 20.0% on days 10, 12, and 14, respectively. In line, significant reduction by 71.6% (n=11) of eGFP expressing CMs after reserpine treatment was revealed by flowcytometry. Reserpine neither reduced EB size nor acted cytotoxic on CMs, while increased numbers of neuronal cells were observed. MEA measurements with reserpine-treated EBs showed lower FP frequencies and weak responsiveness to adrenergic and muscarinic stimulation. Co-application of isoproterenol and reserpine during differentiation partially rescued cardiac development. The developmental inhibition after α- and β-adrenergic blocker application mimicked developmental changes with reserpine and proved an involvement of adrenergic receptors in the process. We therefore conclude that catecholamines and adrenergic signaling play a critical role during cardiac development in ES cells.
Biography
Martin Lehmann has completed his PhD in 2014 from the University of Cologne. He currently works as a Postdoctoral Marie-Curie Fellow for the Institute of Neurophysiology of the University Hospital of the University of Cologne on a EU-granted project, performing parts of his research in a Biotechnology Company in Budapest (Hungary).
image PDF   |   image HTML
 

Relevant Topics

Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2018-19
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

Agri & Aquaculture Journals

Dr. Krish

[email protected]

+1-702-714-7001Extn: 9040

Biochemistry Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Business & Management Journals

Ronald

[email protected]

1-702-714-7001Extn: 9042

Chemistry Journals

Gabriel Shaw

[email protected]

1-702-714-7001Extn: 9040

Clinical Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Engineering Journals

James Franklin

[email protected]

1-702-714-7001Extn: 9042

Food & Nutrition Journals

Katie Wilson

[email protected]

1-702-714-7001Extn: 9042

General Science

Andrea Jason

[email protected]

1-702-714-7001Extn: 9043

Genetics & Molecular Biology Journals

Anna Melissa

[email protected]

1-702-714-7001Extn: 9006

Immunology & Microbiology Journals

David Gorantl

[email protected]

1-702-714-7001Extn: 9014

Materials Science Journals

Rachle Green

[email protected]

1-702-714-7001Extn: 9039

Nursing & Health Care Journals

Stephanie Skinner

[email protected]

1-702-714-7001Extn: 9039

Medical Journals

Nimmi Anna

[email protected]

1-702-714-7001Extn: 9038

Neuroscience & Psychology Journals

Nathan T

[email protected]

1-702-714-7001Extn: 9041

Pharmaceutical Sciences Journals

Ann Jose

[email protected]

1-702-714-7001Extn: 9007

Social & Political Science Journals

Steve Harry

[email protected]

1-702-714-7001Extn: 9042

 
© 2008- 2018 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version
Leave Your Message 24x7