Reach Us +44-1202-068036
Evidence Of Phenylalanine Pathway In Resveratrol-producing Alternaria Sp.: Substrate Flow And Enzyme Activities | 16244
ISSN: 2153-0769

Metabolomics:Open Access
Open Access

Like us on:

OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations
700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)
All submissions of the EM system will be redirected to Online Manuscript Submission System. Authors are requested to submit articles directly to Online Manuscript Submission System of respective journal.

Evidence of phenylalanine pathway in resveratrol-producing Alternaria sp.: Substrate flow and enzyme activities

3rd International Conference and Exhibition on Metabolomics & Systems Biology

Junling Shi

Accepted Abstracts: Metabolomics

DOI: 10.4172/2153-0769.S1.026

Alternaria sp. MG1 is an endophytic fungus isolated from grape and has capability in producing resveratrol, indicating the occurrence of resveratrol biosynthesis pathway in this strain. Different substrates and C 13 -glucose were used in the culture of Alternaria sp. MG1 resting cells. The intermediates accumulated during the culture were identified using high performance liquid chromatography (HPLC) and mass spectrograph. During the biotransformation, activities of related enzymes, including phenylalanine ammonia-lyase (PAL), trans-cinnamate 4-hydroxylase (C4H) and 4-coumarate-CoA ligase (4CL) were analyzed and tracked. The reaction system contained 100 mL 0.2 mol/L phosphate buffer (pH 6.5), 120 g/L Alternaria sp. MG1 cells, 0.1 g/L MgSO 4 and 0.2g/L CaSO 4 and different substrates according to the experimental design. The biotransformation was carried out for 21 hours at 28?C and 120 rpm. Resveratrol formation was identified when phenylalanine, tyrosine, cinnamic acid and p-coumaric acid were separately used as only substrate. Accumulation of cinnamic acid, p-coumaric acid and resveratrol and activities of PAL, C4H and 4CL were identified and changed in different trends during transformation with phenylalanine as only substrate. C 13 isotope analysis showed 1 or 7 carbon atoms were transferred from glucose to the intermediates and substrates in phenylalanine pathway, indicating the occurrence of this pathway in Alternaria sp.
Junling Shi completed her Ph.D. from Northwest Agriculture and Forestry University and postdoctoral studies from Jiangnan University. She is a Professor of Northwestern Polytechnical University now. She has published more than 30 papers in reputed journals. Her major research field is endophytic fungi and fungal metabolism.
Leave Your Message 24x7