alexa Exogenous SiRNA-mediated Protection Of Plants From Virus Infection
ISSN: 1948-5964

Journal of Antivirals & Antiretrovirals
Open Access

Like us on:
OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations

700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

Share This Page

Additional Info

Loading
Loading Please wait..
 

4th World Congress on Virology
October 06-08, 2014 Hilton San Antonio Airport, TX, USA

Yiguo Hong
Accepted Abstracts: J Antivir Antiretrovir
DOI: 10.4172/1948-5964.S1.022
Abstract
A hallmark of antiviral RNA silencing is the production of viral small interfering RNA (vsiRNA). Profiling of vsiRNAs indicates that many regions of viral RNA genome or transcribed viral RNAs are prone to RNA silencing-mediated cleavage. These vsiRNAs could be used to combat virus infection in hosts. Here we show that direct targeting viral genomes by synthetic certain vsiRNAs confers plant resistance to virus infection. Using northern blotting and high-throughput deep sequencing, we obtained vsiRNA profiles for the African cassava mosaic virus (ACMV), a single-stranded DNA virus that causes massive economic losses in the production of cassava (Manihot esculenta Crantz), an essential staple carbohydrate food source in Africa and other countries. Sense and anti-sense strands of small RNAs corresponding to hotspot and coldspot vsiRNAs were synthesised. We found that double-stranded hotspot small RNAs were able to protect Nicotiana benthamiana from ACMV infection. Consequently, viral DNA replication and accumulation of viral mRNA were undetectable and the treated plants remain healthy. However, the sense or anti-sense strand of hotspot vsiRNAs, and coldspot small RNAs in both double-stranded and single-stranded conformations possessed less or no activity in viral protection. We further demonstrated that the exogenous vsiRNA-mediated virus resistance had a threshold effect and required an active RDR6. These data show that vsiRNAs possess a functional significance on antiviral RNA silencing, suggesting that they may have the potential as an exogenous biological agent for controlling destructive viral diseases in plants and other hosts.
Biography
Yiguo Hong obtained his PhD in plant molecular virology in 1990. As a Principal Investigator he had established an independent Molecular Virology Laboratory at Horticultural Research International (HRI), and then Warwick HRI, University of Warwick since 1999 after his postdoctoral research at Imperial College London, John Innes Centre, Norwich and Scottish Crop Research Institute, Dundee in the UK. He is now a Professor and Director of the Research Centre for Plant RNA Signalling in the College of Life and Environmental Sciences, Hangzhou Normal University while he holds a lasting Visiting Professorship at the University of Warwick. His research interests include RNA and protein signalling in antiviral defence and plant development.
image PDF   |   image HTML
 
Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

 
© 2008-2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version
adwords