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Expression Profi Le And Function Analysis Of MiRNAs In Breast Cancer Stem Cells | 5237
ISSN: 2157-7013

Journal of Cell Science & Therapy
Open Access

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Expression profi le and function analysis of miRNAs in breast cancer stem cells

International Conference & Exhibition on Cell Science & Stem Cell Research

Jian-guo Sun, Zheng-tang Chen, Xinxin Wang and Hong Chen

ScientificTracks Abstracts: J Cell Sci Ther

DOI: 10.4172/2157-7013.S1.02

Abstract
Even though breast cancer stem cells (BCSCs) make up less than 2% of breast cancer cells, they are regarded as the root of breast cancer metastasis, recurrence and chemotherapy failure. Recent studies have shown that miRNAs are involved in self-renewal and diff erentiation of stem cells. miRNAs may also involved in the process of mammary epithelium stem cells (MaSCs) mutating into BCSCs. In the present study, we isolated BCSCs (ESA + CD44 + CD24 -/low ) and MaSCs (MUC1 - ESA + ) from MCF-7 and MCF-10A respectively. Th en, microRNA microarray was performed to scan BCSCs-related miRNAs. Th e results have shown that BCSCs have 25 miRNAs and 17 miRNAs of diff erent expression level compared with MCF-7 and MaSCs respectively. We selected several miRNAs for bioinformatic analysis and further study. Using luciferase reporter vector, we demonstrated that miR-21, miR-200c and miR-122a signifi cantly blocked the expression of PDCD4, PDCD10, and G3BP2, respectively. Consideration of expression level tendency, miR-21 (in BASCs > in MaSCs) played an important role as oncogene, miR-122a (in BCSCs > in MCF-7) and miR-200c (in BCSCs < in MCF-7) played important roles as anti-oncogene. Also we found that miR-559 and miR-548d-3p could signifi cantly inhibit HER2 gene, which played an important role in BCSC genesis. In summary, the study identifi ed a series of miRNAs that may regulate BCSCs function, these results may provide molecular basis for the application of miRNAs in breast cancer therapy
Biography
Jian-guo Sun has completed his Ph.D and postdoctoral studies at Third Military Medical University. He is the deputy director of the cancer institute of PLA, Xin-qiao Hospital, Third Military Medical University. His research work focuses on early diagnosis and comprehensive treatment of breast cancer
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