Journal of Clinical and Cellular Immunology
Open Access

Extracellular traps formation of sheep and cattle polymorph nuclear neutrophils against Toxoplasma gondii

Conference Series LLC Joint International Event on 5^th European Immunology & Innate Immunity

July 21-23, 2016 Berlin, Germany

Kader Yildiz, Sami Gokpinar, Aycan Nuriye Gazyagci, Cahit Babur and Ahmet Kursat Azkur

Kirikkale University, Turkey
Turkiye Halk Sagligi Kurumu, Turkey

Posters & Accepted Abstracts: J Clin Cell Immunol

Abstract :

This work was supported by the grand from TUBITAK (Project no: TOVAG 214O288)

Toxoplasma gondii is a zoonotic parasite possesses broad hosts range. Sheep is easily infected with T. gondii and the parasite causes some important pathology dependent on the stage of pregnancy at which the ewe becomes infected; whereas cattle is poor host of the parasite epidemiologically, cattle can be successfully infected with T. gondii, but this parasite is eliminated perhaps due to innate immunity. Innate immune system providing a quick defense against to pathogens enters the body is essential for survivor of multicellular organisms. Primary role of innate immune system is restrict pathogens in infections area and thus prevent to spread into the organism. Innate immunity is a complex mechanism involving several instruments. Neutrophil is major component of innate immune system and fight pathogens with different strategies in organism. One of them, Netosis is an important effector mechanism of the host early immune response against pathogens. The aim of the study was to comparison of neutrophil extracellular traps (NETs) formation of polymorph nuclear neutrophils (PMN) of cattle and sheep confronted with T. gondii tachyzoites. And also aim to determine the effects of incubation time and different parasites ratio on NETs development in cattle and sheep PMN. Sheep and cattle experiments were started at the same day under the same experimental conditions. NETosis was observed in cattle and sheep PMN after confronted with T. gondii tachyzoites in vitro. NETs amount was measured using fluorometer. NETs amount increased depending on tachyzoites intensity and incubation time. Fluorescence microscopic analyses revealed that myeloperoxidase, neutrophil elastase and histones (H3) were detected in NETs structures from sheep and cattle PMN after encounter with T. gondii.

Biography :

Kader Yildiz has completed her PhD studies from Ankara University, Turkey.