alexa
Reach Us +3225889658
Fabrication Of Porous Monolithic Materials Of Reactive Polymers For Protein Purification | 33148
ISSN: 2157-7064

Journal of Chromatography & Separation Techniques
Open Access

Like us on:

OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations
700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)
All submissions of the EM system will be redirected to Online Manuscript Submission System. Authors are requested to submit articles directly to Online Manuscript Submission System of respective journal.

Fabrication of porous monolithic materials of reactive polymers for protein purification

International Conference and Expo on Separation Techniques

Hiroshi Uyama

Osaka University, Japan

Keynote: J Chromatogr Sep Tech

DOI: 10.4172/2157-7064.S1.009

Abstract
Along with the rapid development of diagnosis and proteomic science, the requirement for the purification of biomolecules like proteins, enzymes and nucleic acids becomes extremely urgent. Protein A chromatography and immobilized metal ion affinity chromatography are regarded as the most efficient and promising methods for the protein purification. For these two techniques, the solid support is the crucial part for the protein purification efficiency and specificity. Polymer-based monoliths with tunable bulk and surface properties have attracted considerable attention due to their unique open-cellular three-dimensional porous structure. Recently, we have developed fabrication of such monolithic materials by phase separation of polymer solutions. The present talk deals with functional monoliths based on reactive polymers such as cellulose and poly(vinyl alcohol-co-ethylene) for protein purification. Protein A was introduced on the monolith of these polymers via suitable chemical activation. For an application of immobilized metal ion affinity chromatography, ethylenediaminetetraacetic acid was introduced into these monoliths and nickel ions were located subsequently. IgG and His-tagged proteins were immobilized on these activated monoliths. The protein purification amount and efficiency were evaluated.
Biography

Hiroshi Uyama received his B.S. (1985) and M.S. (1987) from Kyoto University. In 1988, he joined the Department of Applied Chemistry, Tohoku University, as Research Associate and obtained Ph.D. He moved to the Department of Materials Chemistry, Kyoto University in 1997. In 2004, he was appointed as a full professor at the Department of Materials Chemistry, Osaka University. He has published over 270 original papers, 170 book chapters and reviews, and 140 patens.

Email: [email protected]

Top