alexa Fertility Cryopreservation In Oxford, UK
ISSN: 2157-7552

Journal of Tissue Science & Engineering
Open Access

Like us on:
OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations

700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

Share This Page

Additional Info

Loading
Loading Please wait..
 

Joint Conference:3rd International Conference & Exhibition on TISSUE PRESERVATION AND BIOBANKING & 6th International Conference onTISSUE ENGINEERING AND REGENERATIVE MEDICINE
August 23-24, 2017 San Francisco, USA

Jill Davies and Sheila Lane
Oxford University, UK
ScientificTracks Abstracts: J Tissue Sci Eng
DOI: 10.4172/2157-7552-C1-033
Abstract
Late effects of cancer treatment may cause premature infertility in children and adolescent cancer survivors. Oxford applied for UK license for cryopreservation of ovarian and immature testicular tissue (ITT) for patients requiring gonadotoxic treatment. This required validation with animal/human studies, development of protocols, information, staff training, establishing costs/funding source. Oxford recruited first set of patients in 2013. Patients eligible for treatment intent is curative, cytoxicity high risk, age <40 years (females) and boys pre-pubertal. Patients consented prior to day of surgery. Ovarian tissue from 145 females, age 10 months – 39 years cryopreserved (14% with benign diagnosis), testicular tissue (ITT) from 31 boys, (0.9–13.6 years). Referrals from oncologists/hematologists and patients, most patients travel to Oxford for surgery scheduled to coincide with other interventions (minimizing risk of anesthesia). Right ovary procured using laparoscopic oophorectomy, wedge testicular biopsy procured via midline incision. Tissue bank technician collects tissue and dissects within cleanroom, producing ovarian cortical strips (1x1x5mm) containing primordial follicles or testicular segments (2x2x2 mm) containing spermatagonial stem cells. Histological evaluation reviews viability and presence of metastatic cells. Immature oocytes collected for in vitro maturation (IVM) and vitrified. Cryopreservation of ovarian tissue requires ethylene glycol, sucrose, human albumin serum, slow controlled freezing (manual seeding at 8˚C) and vapor phase nitrogen storage. DMSO is used for ITT. Tissue is stored until the patient is cured and confirmed infertile. 86 live births following ovarian tissue transplantation was recorded. A live birth in ITT is successful in animal studies only. Patients with hematological malignancy will use vitrified oocytes (gametes are free from residual disease). For other females and ITT patients, tissue will be matured in vitro (IVM) to develop primordial follicles/spermatogenesis for use in conventional ART. Oxford University researchers currently are optimizing IVM techniques (three dimensional/organ culture studies). All details were registered in OTCP database. Service evaluation (interviews with patients/parents) confirms patients/parents understood risks/potential for fertility restoration. Setting up the Oxford service has been challenging due to evolving UK legislation. Referrals are from all UK centers.
Biography

Jill Davies graduated from Coventry University in 1987 with a degree in Applied Biology. After university studies, she worked for cardiothoracic surgeons Mr. Donald Ross & Sir Magdi Yacoub at the National Heart Hospital in London and that she opened the heart valve bank at the Oxford University Hospital in Oxford, England in 1990. The Oxford bank supplies cardiovascular tissue, corneas for transplant and research and brains & spinal cords for research. It is now also an Oxford Research Center Bio bank. She is also an executive member of the BATB, member of AATB, SLTB, and Society for Cryobiology.

image PDF   |   image HTML
 

Relevant Topics

Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

Agri, Food, Aqua and Veterinary Science Journals

Dr. Krish

[email protected]

1-702-714-7001 Extn: 9040

Clinical and Biochemistry Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Business & Management Journals

Ronald

[email protected]

1-702-714-7001Extn: 9042

Chemical Engineering and Chemistry Journals

Gabriel Shaw

[email protected]

1-702-714-7001 Extn: 9040

Earth & Environmental Sciences

Katie Wilson

[email protected]

1-702-714-7001Extn: 9042

Engineering Journals

James Franklin

[email protected]

1-702-714-7001Extn: 9042

General Science and Health care Journals

Andrea Jason

[email protected]

1-702-714-7001Extn: 9043

Genetics and Molecular Biology Journals

Anna Melissa

[email protected]

1-702-714-7001 Extn: 9006

Immunology & Microbiology Journals

David Gorantl

[email protected]

1-702-714-7001Extn: 9014

Informatics Journals

Stephanie Skinner

[email protected]

1-702-714-7001Extn: 9039

Material Sciences Journals

Rachle Green

[email protected]

1-702-714-7001Extn: 9039

Mathematics and Physics Journals

Jim Willison

[email protected]

1-702-714-7001 Extn: 9042

Medical Journals

Nimmi Anna

[email protected]

1-702-714-7001 Extn: 9038

Neuroscience & Psychology Journals

Nathan T

[email protected]

1-702-714-7001Extn: 9041

Pharmaceutical Sciences Journals

John Behannon

[email protected]

1-702-714-7001Extn: 9007

Social & Political Science Journals

Steve Harry

[email protected]

1-702-714-7001 Extn: 9042

 
© 2008-2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version
adwords