Journal of Proteomics & Bioinformatics
Open Access

First report of Colistin Resistance (MCR-1) Gene in Escherichia coli and Klebsiella pneumoniae Isolated from Clinical Specimens in Khartoum, Sudan

Joint Event on 9^th International Conference and Expo on Proteomics and Molecular Medicine & 9^th International Conference on Bioinformatics

November 13-15, 2017 Paris, France

Nahed Adam and Hisham N Altayb

Sudan University of Science and Technology, Sudan
The National University, Sudan

Posters & Accepted Abstracts: J Proteomics Bioinform

Abstract :

The aim of this study was to detect the presence of mcr-1 gene in clinical isolates of Enterobacteriaceae in Khartoum State, Sudan, at the period between February and July 2016 using 50 consecutive clinical Enterobacteriaceae isolates. Species was identified using standard biochemical tests. Antimicrobial susceptibility was done by using the following antibiotics; ciprofloxacin, gentamicin, co-trimoxazole, cefotaxime, cefuroxime and cefixime. DNA was extracted by using bacterial genomic extraction Kits. For the entire isolated organism, PCR was done to detect mcr-1 by using specific primer. The most commonly isolated organism was E. coli 38 (76%), followed by Proteus spp. 8 (16%), and K. pneumoniae 4 (8%). Males (60%) are more infected than females (40%), and elderly patients 61-90 years (42%) are found to be more susceptible to infection. We also reported highly resistant rate to ciprofloxacin (66%), gentamicin (68%) and cefuroxime (96%). mcr-1 gene was detected in 7 (14%) isolates, mostly in E. coli. We reported for the first time in Sudan the presence of mcr-1 gene in clinical isolates, and the prevalence of this gene is higher when compared to other countries.

Biography :

Nahed Adam Abdalla Jebrel, Alwatania University, college of medical lab. Khartoum, Sudan.