Formulation and evaluation of in situ-ophthalmic gel of Ofloxacinand Diclofenac for reducing the inflammation as well as microbial contamination rate after cataract surgery
5th International Conference and Exhibition on Pharmaceutics & Novel Drug Delivery Systems
March 16-18, 2015 Crowne Plaza, Dubai, UAE

Tambe Daniel Atem, KR Sindhu, Rajashekar Valluru and KA sridhar

Posters & Accepted Abstracts: Pharm Anal Acta

Abstract:

Many conventional ophthalmic formulations like solution, ointment, suspension are available in market;yet ophthalmic drug delivery is most challenging and interesting to many researchers because of poor availability of the solution caused by drainage and dilution from the eye, to overcome this problem in situ gelling system has been prepared. The in situ gel forming systems that are installed as a drop into the eye undergo phase transition a sol to gel in the cul- de sac. The present study describes the formulation and evaluation of in situ gel of Ofloxacin and Diclofenac, based on the concept of gelling agent. Sol to gel system were prepared by utilizing the phase transition property of sodium alginate, a novel ophthalmic gel forming mucoadhesive polymer, which gets converted into gel in the presence of divalent-cations like calcium in lachrymal fluid and HPMC E50and HPMC 4KM which acts as a copolymer and viscosity enhancer.Suitable buffering agents were used to adjust the pH 6.5. The formulations were sterilized in autoclave at 1210 C for 15 min and various evaluation tests as well as stability testing of formulation were carried out to identify the ideal formulation. Further the formulation was evaluated for its anti microbial activity and tested in-vivo on rabbits.