alexa Functional Proteomics: Novel Approach To Biomarker Discovery
ISSN: 2157-7064

Journal of Chromatography & Separation Techniques
Open Access

Like us on:
OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations

700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

Share This Page

Additional Info

Loading Please wait..

International Expo and Conference on Analytrix & HPLC
October 22-24, 2012 DoubleTree by Hilton Chicago-North Shore, USA

Jiri Adamec
ScientificTracks Abstracts: J Chromat Separation Techniq
DOI: 10.4172/2157-7064.S1.005
I t is helpful for early diagnosis, staging, prognosis, and mediating the aggressiveness of viral infections and cancer by providing real-time information to doctor. However, the common detecting methods (i.e. ELISA or flow cytometry) have some drawbacks to reflect the real-time information. For example, the method of ELISA usually took 2~3 days to complete the whole process. Therefore, development of detecting method which has properties such as high throughput and sensitivity is essential. In this report, a rapid and highly sensitive chip has been developed for detecting low concentration molecules or specific rare cells. Unlike time-consuming ELISA and flow cytometry, the advantages of the chip include low-cost, reusability, high sensitivity and easy preparation. This chip is based on durable gold nanostructures on transparent glasses with uniform spacing which having an average nano-particle size and inter-particle gap of 8 nm and 11 nm, respectively. According to results, the optical response of localized surface plasmon resonance (LSPR) is strongly dependent on the chemical/biological molecule binding location. The optical response of LSPR increases when binding molecules are immobilized at the inter-particle spacing. The anti-Human immunoglobulin G molecule was applied as target to detect. Chemical immobilization had been used as bridge between the Human immunoglobulin G molecules and chip. The experimental result indicates that the limit of detection for anti-Human immunoglobulin G molecules is 10 ng/mL. In the next step, we will attempt to detect pathogens or specific rare cells (i.e. circulating tumor cells or cytotoxic T lymphocyte) with special functionalization due to the high sensitivity by using our Au- LSPR chips. Now mass-production of the Au-LSPR chips can be achieved according to our patented microwave-plasma methods. Moreover, a hand held equipment of UV absorption has been designed to replace the large apparatus. We hope the Palm-sized equipment for detecting specific molecules that can be applied to Point-of-care testing in the future.

Kuan-Jiuh Lin is the distinguished professor of department chemistry of National Chung Hsing Universtiy, Taichung, Taiwan. His research fields focused on the exploring interfacial electric-materials, such as nanoparticles embedded on glass substrates and photoanode solar cells. He has published more than 100 papers in reputed journals and over 30 patents.

image PDF   |   image HTML
Peer Reviewed Journals
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

© 2008-2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version