hree-dimensional porous structures (scaffolds) have been considered in reconstruction of bone defects. The development of
new biomaterials and techniques for obtaining this type of material has provided relevant results and promising for tissue
engineering. The objective of this study was to develop three-dimensional polyhydroxybutyrate structures from rapid prototyping
and functionalized with bacterial cellulose (BC) + osteogenic growth peptide (OGP) for bone regeneration. The scaffolds were
produced by selective laser sintering with pores of 700 μm and 2-mm thickness. Afterwards samples were covered by BC in static
culture. Subsequently to the BC purification, the OGP peptide was incorporated to the scaffolds. The scaffolds were characterized
by scanning electron microscopy (SEM), thermogravimetric analysis (TG/DTA), Raman Scattering and mechanical tests. In vitro
assays evaluated cellular morphology, cell viability, alkaline phosphatase activity (ALP) and mineralized matrix formation. The
OGP peptide influenced osteogenic cell proliferation and favored the mineralization process, conferring osteoinductive property
to PHB/BC scaffolds. The results suggest that scaffolds are potential material for bone tissue engineering/regeneration.
Sybele Saska is dentistry and has completed her Ph.D at the age of 30 years from Univ. Estadual Paulista-UNESP, Institute of Chemistry (Araraquara/
Brazil) with emphasis in biotechnology and postdoctoral studies from Univ. Estadual Paulista-UNESP, Institute of Chemistry in the research line
tissue engineering. She has published more than 15 papers in journals and has 2 patents.
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