alexa Functions Of Epidermal Growth Factor In Human Keratinocytes
ISSN: 2155-9554

Journal of Clinical & Experimental Dermatology Research
Open Access

Like us on:
OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations

700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

Share This Page

Additional Info

Loading
Loading Please wait..
 

3rd International Conference on Clinical & Experimental Dermatology
April 15-17, 2013 Hilton Chicago/Northbrook, USA

Miroslav Blumenberg
ScientificTracks Abstracts: J Clin Exp Dermatol Res
DOI: 10.4172/2155-9554.S1.010
Abstract
One of the challenges of OMICS research is the integration of new data into the pre-existing, and then re-interpretation of the integrated data. We used readily available metaanalysis computational methods to integrate new data on the transcriptomic effects of EGF in primary human epidermal keratinocytes with the preexisting transcriptomics data in keratinocytes. We separately addressed the consequences of adding EGF to keratinocyte cultures and its reverse, blocking the EGFR kinase with Tyrphostin AG1478. We first starve primary human epidermal keratinocytes for 24 hrs and next treated them with EGF. Then, we compared the genes expressed in the treated and control cultures in parallel, using Affymetrix microarrays. We find that the addition of EGF promotes keratinocyte proliferation, attachment and motility and, surprisingly, induces DUSPs, the phosphatases that attenuate the EGF signal. Using meta-analysis, we identified overlapping effects of EGF with those of IL-1 and IFNg, both activators of keratinocyte in wound healing and inflammation. We also identified and characterized the genes and pathways which are suppressed by EGF but are induced by agents promoting epidermal differentiation, such as Ephrins and JNK inhibitors. EGFR activation is important in many malignancies, including cutaneous SCC, lung, colon and other cancers, and targeting the EGFR is currently used to treat such cancers. However, a significant drawback to EGFR targeted therapies is the skin toxicity side effect. This toxicity usually presents as hair and nails abnormalities, papular or pustular folliculitis and pruritic dry skin. These limit the usefulness of EGFR targeting therapies. Surprisingly, the transcriptional effects of EGFR inhibition have not been extensively explored in epidermal keratinocytes. Therefore, we treated primary human epidermal keratinocytes with Tyrphostin AG1478, a specific inhibitor of the EGFR kinase, and compared the treated and control cultures using Affymetrix microarrays. The observed changes were integrated with the pre-existing data on transcriptional profiling in epidermal keratinocytes. We found that the inhibition of EGFR suppresses the transcription of genes linked to keratinocyte proliferation, attachment and motility. Interestingly, inhibiting EGFR promotes apoptosis by both induction of proapoptotic and suppression of antiapoptotic genes. Certain transcriptional effects of EGFR inhibition counter the transcriptional effects of retinoids. Surprisingly, EGFR inhibition strongly and specifically induces expression of markers of epidermal differentiation. Overall, our work defines the yin-yang of EGF signaling in human epidermis, namely both the changes responding to activation of the EGF receptor, and its inhibition. Moreover, this work can serve as a paradigm for integration and analysis of new omics data with the large bodies of data in public repositories.
Biography

Miroslav Blumenberg is an Associate Professor in the R.O. Perelman Department of Dermatology and Department of Biochemistry and Molecular Pharmacology, NYU Langone Medical Center. He has completed his Ph.D. at the Massachusetts Institute of Technology and postdoctoral studies at Stanford University. He has published more than 100 papers in refereed journals, serves on editorial boards of BMC Genomics, Acta Dermatovenerologica APA and World Journal of Biological Chemistry and holds 3 patents. Dr. Blumenberg pioneered the use of DNA microarrays in skin biology.

image PDF   |   image HTML
 

Relevant Topics

Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

Agri & Aquaculture Journals

Dr. Krish

[email protected]

1-702-714-7001Extn: 9040

Biochemistry Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Business & Management Journals

Ronald

[email protected]

1-702-714-7001Extn: 9042

Chemistry Journals

Gabriel Shaw

[email protected]

1-702-714-7001Extn: 9040

Clinical Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Engineering Journals

James Franklin

[email protected]

1-702-714-7001Extn: 9042

Food & Nutrition Journals

Katie Wilson

[email protected]

1-702-714-7001Extn: 9042

General Science

Andrea Jason

[email protected]

1-702-714-7001Extn: 9043

Genetics & Molecular Biology Journals

Anna Melissa

[email protected]

1-702-714-7001Extn: 9006

Immunology & Microbiology Journals

David Gorantl

[email protected]

1-702-714-7001Extn: 9014

Materials Science Journals

Rachle Green

[email protected]

1-702-714-7001Extn: 9039

Nursing & Health Care Journals

Stephanie Skinner

[email protected]

1-702-714-7001Extn: 9039

Medical Journals

Nimmi Anna

[email protected]

1-702-714-7001Extn: 9038

Neuroscience & Psychology Journals

Nathan T

[email protected]

1-702-714-7001Extn: 9041

Pharmaceutical Sciences Journals

Ann Jose

[email protected]

1-702-714-7001Extn: 9007

Social & Political Science Journals

Steve Harry

[email protected]

1-702-714-7001Extn: 9042

 
© 2008- 2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version
adwords