alexa Generation And Characterization Of Brain-specific UBIAD1-deficient Mice Using A Nestin Promoterdriven Cre/loxP Strategy | 20802
ISSN: 2155-9570

Journal of Clinical & Experimental Ophthalmology
Open Access

Like us on:

OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations
700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

4th International Conference on Clinical & Experimental Ophthalmology

Kimie Nakagawa
ScientificTracks Abstracts: J Clin Exp Ophthalmol
DOI: 10.4172/2155-9570.S1.017
Abstract
UBIAD1is a key enzyme of vitamin K2/menaquinone-4 (MK-4) biosynthesis in mammals. Both MK-4 and UBIAD1 distributeatrelatively high levels in brain. However, the roles of UBIAD1and MK-4 in brain functionare not fully understood. We generated mice completely lacking ubiad1 , a homolog of human UBIAD1 by gene targeting; however, they were embryonic lethal. Therefore, to investigate the role of ubiad1 in brain, we conducted a conditional, region-specific, genetic approach to target ubiad1 using the Cre/loxP strategy with a nestin promoter-driven cre transgenic mouse line that will make possible to induce recombination in the central nervous system (CNS). The resulting ubiad1 conditional knockout (CKO) mice were viable, but the size of ubiad1 -CKO mice was significantly smaller than wild-type littermates. UBIAD1 expression and MK-4 concentration of ubiad1 -CKO mice were severely decreased in all brainregions. Ubiad1-CKO mice can be useful for analysis of neuronal function of ubiad1 and MK-4. To address the impact of loss of UBIAD1 signaling in brain, we subjected ubiad1 -CKO mice to multiple behavioral tests. In the open field test, activity levels were higher in ubiad1 -CKO mice than wild-type (WT) littermate. In the Y-maze test, ubiad1 -CKO mice displayed a significantly lower working memory index but higher locomotion activity compared to their WT littermates. Ubiad-CKO mice exhibit decreased anxiety-like behavior. These results indicate that UBIAD1/ ubiad1 or MK-4 may play a critical role in the CNS. Future studies are required to clarify the neurochemical functions of UBIAD1 and MK-4 in brain.
Biography
Kimie Nakagawa received a Ph.D. in Pharmaceutical Sciences from Kobe Pharmaceutical University in 2006 and she is serving as an Associate Professor of the Department of Hygienic Sciences at Kobe Pharmaceutical University since 2012. She has made significant contributions to the identification and characterization of the vitamin K2/MK-4 biosynthetic enzyme, UBIAD1. Her major interests are to generate various tissue-specific UBIAD1-deficient mice in order to explore the physiological and pathophysiological roles of UBIAD1.
image PDF   |   image HTML
 
Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2018-19
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

Agri & Aquaculture Journals

Dr. Krish

[email protected]

+1-702-714-7001Extn: 9040

Biochemistry Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Business & Management Journals

Ronald

[email protected]

1-702-714-7001Extn: 9042

Chemistry Journals

Gabriel Shaw

[email protected]

1-702-714-7001Extn: 9040

Clinical Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Engineering Journals

James Franklin

[email protected]

1-702-714-7001Extn: 9042

Food & Nutrition Journals

Katie Wilson

[email protected]

1-702-714-7001Extn: 9042

General Science

Andrea Jason

[email protected]

1-702-714-7001Extn: 9043

Genetics & Molecular Biology Journals

Anna Melissa

[email protected]

1-702-714-7001Extn: 9006

Immunology & Microbiology Journals

David Gorantl

[email protected]

1-702-714-7001Extn: 9014

Materials Science Journals

Rachle Green

[email protected]

1-702-714-7001Extn: 9039

Nursing & Health Care Journals

Stephanie Skinner

[email protected]

1-702-714-7001Extn: 9039

Medical Journals

Nimmi Anna

[email protected]

1-702-714-7001Extn: 9038

Neuroscience & Psychology Journals

Nathan T

[email protected]

1-702-714-7001Extn: 9041

Pharmaceutical Sciences Journals

Ann Jose

[email protected]

1-702-714-7001Extn: 9007

Social & Political Science Journals

Steve Harry

[email protected]

1-702-714-7001Extn: 9042

 
© 2008- 2018 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version
Leave Your Message 24x7