alexa Genetic Code Expansion In Natural Propagation For Site-Specific Engineering And Tracking Of Single Adeno-Associated Viruses
ISSN: 1948-5964

Journal of Antivirals & Antiretrovirals
Open Access

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4th World Congress on Virology
October 06-08, 2014 Hilton San Antonio Airport, TX, USA

Chuanling Zhang, YongxiangZheng, Fei Yu, Yiming Wu, Longlong Shi, Qing Xia, Sulong Xiao, Maorong Yu, Qiuchen He, Lihe Zhang and Demin Zhou
ScientificTracks Abstracts: J Antivir Antiretrovir
DOI: 10.4172/1948-5964.S1.020
With the aim of studying structure-function relationships of viruses,we developed a general approach that directed azide-bearing amino acids site-specifically to any defined position ofprogeny adeno-associated viruses (AAV)via expanded genetic codes. As a demonstration and application, azides embeddedat AAVcapsids are subsequently coupled withfluorophoresbioorthogonallyin high efficiency, generating visible viruses withprobes incorporated at optimal sites that cause minimal disturbance on viral propagation and infection. The facile and mild realization of site-specific engineering of AAVby natural propagation is of considerable interest to both basic research and for therapeutic applications.
Chuanling Zhang has completed his PhD at the age of 28 years from PekingUnion Medical Collegeand worked at Peking University Health Science Center as an assistant professor. He has published more than 10 papers in reputed journals.
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