alexa Genotyping Of Mycobacterium Bovis Isolated At Human-animals Interface Of The Serengeti Ecosystem In Northern Tanzania
ISSN: 2155-9597

Journal of Bacteriology & Parasitology
Open Access

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2nd International Congress on Bacteriology & Infectious Diseases
November 17-19, 2014 DoubleTree by Hilton Hotel Chicago-North Shore, USA

Bugwesa Zablon Katale
Accepted Abstracts: J Bacteriol Parasitol
DOI: 10.4172/2155-9597.S1.008
Interspecies transmission of tuberculosis at human-animal interface areas is of particular concern, particularly in areas where human, livestock and wildlife live in close proximity. This study aimed at investigating the species diversity of Mycobacterium bovis at human livestock-wildlife interface areas and possibility of M. bovis interspecies transmission. Sputum samples from 421 suspect TB patients attended health services in Bunda, Serengeti and Ngorongoro districts hospitals, Tanzania were collected between 2010-2013. Livestock and wild animals? tissues were collected from slaughter houses and wildlife protected areas respectively. Culture isolates were characterized using spoligotyping and MIRU-VNTR. Analysis and interpretation of genotypic results was performed using Mycobacterial spoligo data base, MIRU-VNTR plus. Based on spoligotyping, M. bovis strains belong to SB0133 and 2 novel strains were identified. No any M. bovis was isolated from tuberculosis patients. There was 100% genetic relatedness amongst strains of M. bovis isolated from wildlife species. The spoligotypeSB0133, which was dominant in wildlife species, was 96.8% and 45.2% genetic agreement to those found in livestock. There is close genetic agreement between M. bovis isolates circulating in wildlife and one of the isolates from livestock suggesting for possibility of M. bovis crossing over between hosts. It appears that, M. bovis is emerging from wildlife reservoirs and become established in livestock where it has genetically changed, suggesting for possibility of wild animals species as source of M. bovis infection in cattle.Therefore, diseases control programs should be directed on minimizing contact among M. bovis hosts at human-animal interface areas.
Bugwesa Zablon Katale has completed his PhD at Muhimbili University, Tanzania. He is a research scientist working with Tanzania Wildlife Research Institute, Ministry of Tourism and Natural Resources, Tanzania. His research interest is on tuberculosis cross transmission at human-animal interface areas.He has published more than ten papersin reputed international journals.
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