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|Svetla Gateva, Gabriele Jovtchev, Alexander Stankov and Milka Mileva|
Stephan Angeloff Institute of Microbiology, Bulgaria
|Posters & Accepted Abstracts: Nat Prod Chem Res|
|Rosa alba L. is a rich source of natural antioxidants and holds promise to be successfully used in perfumery, cosmetics and pharmacy. The natural monoterpene geraniol comprises 18.28% of the Rosa alba L. essential oil. The compound was tested with respect to its potential to protect cells against DNA alkylating agent N-methyl-N’-nitro-N-nitrosoguanidine (MNNG), as well as to its cytotoxic and genotoxic activities in two types of test-systems: Hordeum vulgare and human lymphocytes in vitro. Such studies with physiologically different types of test-systems could provide more informative and representative assessment about the cytotoxic/genotoxic effect and protective potential of the compounds isolated from white rose essential oil against genotoxins. The objective of the study was to evaluate cytotoxic/clastogenic effect and anti-cytotoxic/anti-clastogenic potential of geraniol (ethanolic extract) evaluated by chromosome aberration test in barley (reconstructed karyotype MK14/2034) and cultured human lymphocytes. Two types of experimental schemes were applied: single treatment with compounds, conditional treatment with geraniol prior to challenge with MNNG with or without 4 h inter-treatment time. The presented data reveals no cytotoxic effect in all geraniol individual treatment variants compared with untreated control. Geraniol shows anti-clastogenic potential against MNNG induced chromosomal aberrations in both test-systems. It is effective in concentration range of 25, 50 and 100 μg/ml and shows a substantial impact after conditional treatment prior to MNNG (50 μg/ml) without or with 4h intertreatment time. The frequency of chromosome aberrations was decreased in dependence of the concentration applied and the treatment variant. 34-60% less injury was detected in lymphocytes and 53-75% in barley compared with the challenging treatment with MNNG alone. These investigations, concerning the anti-cytotoxic and anti-genotoxic potential of geraniol could be useful in health research on the pharmacological capacity and activity of the present and other natural plant compounds.|
Svetla Gateva has experience in the field of genotoxicity screening and risk assessment of various environmental contaminants using human lymphocytes as a test-system. She has experience with classical cytogenetic techniques (chromosome aberrations, micronuclei) and molecular methods (comet analysis and gel electrophoresis in a constant electric field/CFGE).
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