alexa Goat Milk Polyunsaturated Fatty Acids Determination By Gas Chromatography
ISSN: 2157-7064

Journal of Chromatography & Separation Techniques
Open Access

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4th World Congress on Chromatography
August 07-09, 2017 | Rome, Italy

Mariana Ropota, Margareta Olteanu, Ilie Voicu and Angela Sava
INCDBNA-IBNA, Romania
USAMV-Bucharest, Romania
Posters & Accepted Abstracts: J Chromatogr Sep Tech
DOI: 10.4172/2157-7064-C1-032
Abstract
The differences in the fatty acids content of the goat milk were determined by gas chromatography in two groups of Carpatina×Saanen (F3) goats (n=30), in the same physiological state (second lactation), fed from April to August according to the intensive system (E1 indoor) or extensive system (E2 indoor + pasture). Group 1 received briquettes made of hays and concentrates, alfalfa hay and barley straws. Group 2 received a supplement of alfalfa hay plus concentrates (corn and barley). Ten milk samples from each group were collected monthly. The method involved the chemical processing of the samples by transformation in fatty acids methyl esters (FAME), followed by separation of the components in the chromatographic column, fatty acids identification by comparison with standard chromatograms and quantitative determination of FAME (g FAME of % g Total FAME). Reference materials (CRM) were: Standard solution of methyl fatty acids SUPELCO 37 Component FAME Mix; 10mg/mL and; infant/adult nutritional formula- standard reference material 1849. The method was validated “in house”, determining the following parameters: accuracy, fidelity, reproducibility, sensitivity, detection limit, quantification limit and tracing in agreement with SR EN ISO/CEI 17025:2005. The experimental determinations showed that Ω6/Ω3 ratio ranged from 7.93 to 12.44 in E1, and from 3.62 to 8.32 in E2. CLA (conjugated linoleic acid) level increased in the middle period of sampling (months 2, 3 and 4), from 0.52% to 0.68% in group 1, and from 0.35% to 0.80% in group 2. In the first and last months, CLA level decreased from 0.57% to 0.53% in group 1, and from 0.63% to 0.52% in group 2, but the average CLA value for the whole experimental period was 6.02% in group 2, compared to just 5.7% in group 1.
Biography

Mariana Ropota completed her PhD at Bucharest University. She is coordinating the compartment of gas chromatographic analyses within the laboratory of chemistry and nutrition physiology of the National Research-Development Institute for Animal Biology and Nutrition-IBNA-Balotesti. She is a Researcher in Analytical Chemistry. She has published more than 20 papers in national and international scientific journals, rated by ISI or by other databases.

Email: [email protected]

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