alexa Graphene-based Signal-enhanced In Vitro Diagnostic Kits For Healthcare Applications | 24716
ISSN: 2169-0022

Journal of Material Sciences & Engineering
Open Access

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3rd International Conference and Exhibition on Materials Science & Engineering

Sandeep K Vashist
Accepted Abstracts: Material Sci Eng
DOI: 10.4172/2169-0022.S1.017
Abstract
Graphene has been widely used in biosensor and bioanalytical applications due to its high electrical and thermal conductivity, large surface area, high mechanical strength, low cost and absence of metallic impurities. During the last decade, graphene has been extensively employed for electrochemical sensing of blood glucose and other analytes. But the recent years have seen its use in diverse bioanalytical applications. We have used graphene in the development of signal-enhanced in vitro diagnostic (IVD) kits for various disease biomarkers such as human lipocalin 2, human fetuin A and C-reactive protein. The developed IVD kits have many-fold higher analytical sensitivity, significantly reduced immunoassay duration, and highly improved analytical performance in comparison to the commercial IVD kits. They employ a highly simplified and cost-effective bioanalytical procedure involving our proprietary one-step antibody immobilization procedure and one-step kinetics-based immunoassay. They have high analytical precision, as demonstrated by correlation with commercial IVD kits, and can detect biomarkers in complex sample matrices, such as human whole blood, plasma and serum. Moreover, the antibody-bound graphene-functionalized microtiter plates have high storage stability, as desired for the clinical settings. Therefore, they can be reliably employed for the highly-sensitive and rapid detection of disease biomarkers in clinical diagnosis.
Biography
Sandeep K Vashist completed his PhD from Central Scientific Instruments Organisation, India in 2006. He was Scientist at Bristol-Myers Squibb Company, Ireland (2006-2009), Team Leader at NUS Nanoscience and Nanotechnology Initiative, Singapore (2009-2012) and presently, the Head of Immunodiagnostics at HSGIMIT, Germany. His outputs include many technology transfers, 5 PCT stage patents, 60 publications in reputed journals, and 70 publications in international conferences. He has received prestigious fellowships and awards from renowned institutions for scientific excellence. He is Executive Editor of J Basic Appl Sci and J Pharma Bioanal Sci; Editorial Board Member of J Nanomed Nanotechol and J Nanomat Mol Nanotechnol, and expert reviewer for many journals and funding agencies.
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