alexa Heat Exposures Increase Glucose Uptake Via GLUT-1 Expression In Mouse Skeletal Muscle
ISSN: 2155-6156

Journal of Diabetes & Metabolism
Open Access

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14th International Conference and Exhibition on Targeting Diabetes and Novel Therapeutics
October 17-18, 2016 Kuala Lumpur, Malaysia

Duhita Pramesthi H
Airlangga School of Medicine, Indonesia
Posters & Accepted Abstracts: J Diabetes Metab
DOI: 10.4172/2155-6156.C1.058
Abstract
Excersice is known as an important way to maintain the normal blood glucose. But many of diabetes mellitus (DM) patients cannot do the excersice well. Heating therapy is predicted as one of the best alternative for excersice supplement. On the other hand, when body get a stressor, GLUT-1 will be expressed. So, we want to know the relationship beetwen lowering blood glucose by heating therapy method and GLUT-1 expression. Mice were divided into four different temperature treatment: 20°C, 27°C, 37°C and 40°C. Each group was consisted of seven mices. The mices were fasted for 16 hours then fasting blood sugar was measured. Then each mice was given glucose 0.2 gram and 30 minutes later blood sugar was re-measured. Then mice incorporated into the heating therapy box and were treated for 30 minutes. Gastrocnemius musculus of the mice was taken and then coloured by immunohistocimia and GLUT-1 antibody to measure the activity of GLUT-1. GLUT-1 expression was best in 40°C but worst in 37°C. Best result of glucose uptake in 37°C was caused by the effective expression of GLUT-4. In diabetic people, there is a disturbance of GLUT-4 activity. On the other hand, the expression of GLUT-1 is not related to insluin activity. So according to these research, in hipertermy condition (40°C), the expression of GLUT-1 will help the glucose uptake in people with disturbance in GLUT-4 expression.
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