alexa Hedera Nepalensis: A Novel Source Of Natural Cancer Chemopreventive And Anticancerous Compounds
ISSN: 1948-5956

Journal of Cancer Science & Therapy
Open Access

Like us on:
OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations

700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

Share This Page

Additional Info

Loading Please wait..

4th World Congress on Cancer Science & Therapy
October 20-22, 2014 DoubleTree by Hilton Hotel Chicago-North Shore Conference Center, USA

Laila Jafri, Samreen Saleem, Ihsan-ul-Haq, Tamara P Kondrytuk, John M Pezzuto and Bushra Mirza
Accepted Abstracts: J Cancer Sci Ther
DOI: 10.4172/1948-5956.S1.035
Traditionally medicinal plants are used for prevention as well as for the treatment of several diseases. Considering the medicinal importance of Hedera nepalensis based on traditional information, the present study was undertaken to analyze in vitro cancer chemopreventive and anti-proliferative sulforhodamine B (SRB) properties of the plant and a compound isolated from it i.e. lupeol. The in vitro cancer chemopreventive testing was performed using quinone reductase (QR) induction assay, inhibition of TNF-α activated nuclear factor kappa-B (NFκB assay), Inhibition of lipopolysaccharide (LPS)-activated nitric oxide (NO) production in murine macrophage RAW 264.7 cells (iNOS assay) and aromatase inhibition assay by crude extract and its three fractions (n-hexane, ethyl acetate and aqueous). The anti-proliferative assay was evaluated on three types of cancer cell lines: MCF-7, MDA-MB-231 andHeLausing 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) reduction assay. The results of cancer chemopreventive assays show thatn-hexane and ethyl acetate fractions of tested plant were having promising cancer chemopreventive potential while lupeol has shown lowest IC50 (0.20 μM/ml) in NFκB assay.Crude extract and its fractions inhibited the growth of three cancer cell lines by more than 60% while IC50 value of lupeol varied from 2.32-10.2 μM/ml. HPLC-DAD based quantification of lupeol in different plant tissues demonstrated that only leaves of H. nepalensis contain lupeol (0.196 mg/100 mg dry weight). In the light of the data generated the novel properties of H. nepalensis harboring cancer chemopreventive and anti-proliferative agents have been discussed.
Laila Jafri (age of 31 years) is PhD student of biochemistry Department, Quaid-i-Azam university Islamabad Pakistan. She has published 2 papers and 4 papers are submitted in well reputed journals.
image PDF   |   image HTML

Relevant Topics

Peer Reviewed Journals
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

© 2008-2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version