alexa Hepatoprotective Effect Of The Crude Flavonoid Extract Of Bay Leaves Extract (Laurus Nolbilis (Lauraceae)) On Primary Cultured Rat Hepatocytes | 13783
ISSN: 2167-7689

Pharmaceutical Regulatory Affairs: Open Access
Open Access

OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations
700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

3rd International Conference on Pharmaceutical Regulatory Affairs

Sahar A. Hussein, Nesrine M. Hegazi, Amani N. Hashim and Nahla A. Ayoub
Posters: Pharmaceut Reg Affairs
DOI: 10.4172/2167-7689.S1.011
Abstract
Background: The crude flavonoid extract of bay leaves was tested for its hepatoprotective activity on primary cultured rat hepatocytes against paracetamol toxicity. The neutral red assay was used to assess the cytotoxicity of the extract and was applied with a broad range of concentrations (from 125-1000 μg/ml) on monolayer of rat hepatocytes. It revealed that the extract exerts no toxic effect on the monolayer hepatocyte layer. Materials and Methods: For the evaluation of the hepatoprotective effect, different concentrations were prepared, starting from 12.5 μg/mL and increasing concentration in ascending order by dissolving in DMSO (1% maximum concentration). For each concentration, three replicates were carried out, in addition to controls which were: cell control (cells only), negative control (cells + paracetamol) and positive control (reference) (cells + sylimarin + paracetamol). Results: Mortality of the hepatocyte (IC50) was determined using neutral red assay and L. nobilis extract showed hepatoprotective activity against paracetamol toxic effect at 40 μg/ ml. The constitutive flavonoids of bay leaves were extensively studied and led to the separation and identification of 14 phenolic compounds which were identified using chemical, conventional and advanced NMR, 1-D and2-D spectral techniques. Conclusion: From the point of view of the main classes of natural products existing in the leaves Laurus nolbilis ( Lauraceae) and on the basis of the analytical results achieved during the course of the present work we can come to the conclusion that showed hepatoprotective activity is produced by the leaf extract of the plant could be due to the existing combination of phenolic constituents or it could be attributed to one or more of the phenolic compounds and the (IC50) of the different fractions suggested that the antioxidant activity is best interpreted in terms of the existing phenolics, catchine, epicatchine, rutin, other flavonoid glycosides and chlorogenic acid which are well known for their potent antioxidant activities and corresponding significant inhibition of UV induced IL-6 production.
image PDF   |   image HTML
 

Relevant Topics

Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2018-19
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

Agri & Aquaculture Journals

Dr. Krish

[email protected]

+1-702-714-7001Extn: 9040

Biochemistry Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Business & Management Journals

Ronald

[email protected]

1-702-714-7001Extn: 9042

Chemistry Journals

Gabriel Shaw

[email protected]

1-702-714-7001Extn: 9040

Clinical Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Engineering Journals

James Franklin

[email protected]

1-702-714-7001Extn: 9042

Food & Nutrition Journals

Katie Wilson

[email protected]

1-702-714-7001Extn: 9042

General Science

Andrea Jason

[email protected]

1-702-714-7001Extn: 9043

Genetics & Molecular Biology Journals

Anna Melissa

[email protected]

1-702-714-7001Extn: 9006

Immunology & Microbiology Journals

David Gorantl

[email protected]

1-702-714-7001Extn: 9014

Materials Science Journals

Rachle Green

[email protected]

1-702-714-7001Extn: 9039

Nursing & Health Care Journals

Stephanie Skinner

[email protected]

1-702-714-7001Extn: 9039

Medical Journals

Nimmi Anna

[email protected]

1-702-714-7001Extn: 9038

Neuroscience & Psychology Journals

Nathan T

[email protected]

1-702-714-7001Extn: 9041

Pharmaceutical Sciences Journals

Ann Jose

[email protected]

1-702-714-7001Extn: 9007

Social & Political Science Journals

Steve Harry

[email protected]

1-702-714-7001Extn: 9042

 
© 2008- 2018 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version
Leave Your Message 24x7