alexa High Performance Liquid Chromatography Fingerprinting Technology For Herbal Drugs
ISSN: 2167-0501

Biochemistry & Pharmacology: Open Access
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International Conference on Pharmacognosy, Phytochemistry & Natural Products
October 21-23, 2013 Radisson Blu Plaza Hotel, Hyderabad, India

Shilpa Rohilla and Priti Jain
Accepted Abstracts: Biochem & Pharmacol
DOI: 10.4172/2167-0501.S1.004
A chromatographic fingerprint of a herbal drug is a chromatographic pattern of pharmacologically active and chemically characteristic constituents present in the extract. But the quality control and quality assurance still remains a challenge because of the high variability of chemical components involved and creates problem in establishing quality. Based on the concept of phytoequivalence, the high performance liquid chromatography (HPLC) fingerprints of herbal medicines could be utilized. With the help of the spectral information the hyphenated instruments show greatly improved performances in terms of the elimination of instrumental interferences, retention time shift correction, selectivity, chromatographic separation abilities and measurement precision. In order to obtain better separation, new techniques have been recently developed in research field of liquid chromatography such as reversed-phase ion-pairing HPLC (RP-IPC-HPLC), strong anion exchange HPLC (SAXHPLC). Due to complexity of the chromatographic fingerprint and the irreproducibility of chromatographic instruments and experimental conditions, several chemometric approaches such as variance analysis, peak alignment, correlation analysis, and pattern recognition are employed to deal with the chromatographic fingerprint.
Shilpa Rohilla has completed her B.Pharm from Pt. B. D. Sharma,UHS,Rohtak and doing her M. Pharm specialisation in Pharmaceutical Chemisry from Birla Institute of Technology And Sciences.
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