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Holy Cow-reshaping Bovine Antibody Diversity With Disulfide-bonded Ultralong CDR H3s | 9106
ISSN: 2157-7579

Journal of Veterinary Science & Technology
Open Access

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Holy cow-reshaping bovine antibody diversity with disulfide-bonded ultralong CDR H3s

International Conference on Animal & Dairy Sciences

Vaughn V. Smider

AcceptedAbstracts: J Veterinar Sci Technolo

DOI: 10.4172/2157-7579.S1.004

Cows have limited genome encoded combinatorial diversity potential in their immune system, yet mount a robust antibody response. Cows havefew V-regions but exceptionally long CDR H3 loops, however the mechanism for creating diversity is not understood. Crystal structures of two cow antibodies reveal that these CDR H3s form a very unusual architecture composed of a β -strand ?stalk? that supports a structurally diverse, disulfide-bonded, ?knob? domain. Deep sequencing revealed that ultralong CDR H3s contain a remarkable complexity of cysteines, suggesting that these disulfide-bonded mini-domains may arise during repertoire development. Sequence analysis indicates that diversity arises from somatic hypermutation of an ultralong DH with a severe codon bias towards mutation to cysteine. These unusual antibodies can be elicited to recognize defined antigens through the knob domain. Thus, the bovine immune system produces an antibody repertoire composed of CDR H3s of unprecedented length that fold into a diversity of mini-domains generated through combinations of somatically generated disulfides.