alexa
Reach Us +1-504-608-2390
Hyaluronidase, Hyaluronan And Inflammation: Answers From The Air Pouch | 19635
ISSN: 2155-9899

Journal of Clinical & Cellular Immunology
Open Access

Like us on:

OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations
700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

3rd International Conference and Exhibition on Clinical & Cellular Immunology

John Huang
ScientificTracks Abstracts: J Clin Cell Immunol
DOI: 10.4172/2155-9899.S1.017
Abstract
Hyaluronan (HA) is involved in many biological activities, such as inflammation, angiogenesis, and tumorigenesis. Some activities are associated with specific sizes of HA molecules, the degradation intermediates of hyaluronidases, such as PH20. This enzyme cleaves HA and generates lower molecular weight (LMW) HA, which was reported to stimulate the innate immune response. However, most PH20 enzymes used were protein extracts from animal tissues, e.g., bovine testis-derived hyaluronidase (BTH), and prone to artifacts due to impurity (including endotoxin). We tested a highly purified recombinant human hyaluronidase, rHuPH20, in the air pouch model of inflammation. In our study, rHuPH20 degraded HA to LMW fragments ranging from 50-500 kDa. While lipopolysaccharide (LPS) stimulated many cytokines and chemokines, rHuPH20 did not stimulate any cytokines and chemokines, suggesting that generation of LMW HA in situ does not stimulate cytokine and chemokine production. LPS also induced neutrophil infiltration into the air pouch, which was not observed with rHuPH20 treatment. Exogenous LMW HA failed to stimulate either cytokines and chemokines or neutrophil infiltration. BTH contain endotoxin and stimulated a strong inflammatory response, which was largely reduced by endotoxin removal. When dosing rHuPH20 together with LPS, the profiling of cytokine and chemokine stimulation was the same as LPS treatment, but neutrophil infiltration was inhibited. The rHuPH20-mediated anti-neutrophil infiltrating effect was also observed in LTA- induced neutrophil infiltration. In conclusion, our results indicate that neither rHuPH20 nor LMW HA has inflammatory properties, and instead, rHuPH20 can inhibit some aspects of inflammation, such as neutrophil infiltration into the air pouch
Biography
John Huang has his career as a drug development biologist in pharmaceutical companies, focusing on oncology, metabolic disease and immunology. Currently, he is working at Halozyme Theraprutics Inc., leading the cell biology group as Associate Director. His research and preclinical work focus on understanding the pharmacological mechanism of action of PH20, as well as exploring hyaluronan biology in all applied disease areas. He was a senior principal scientist at Pfizer Inc., a senior Scientist at Receptor Biologix Inc., following being a Scientist at Amgen Inc. He graduated with honors from the University of California Riverside, and had his Postdoc training at UCSF with Dr. J Michael Bishop.
image PDF   |   image HTML
 

Relevant Topics

Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2018-19
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

Agri and Aquaculture Journals

Dr. Krish

[email protected]

+1-702-714-7001Extn: 9040

Biochemistry Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Business & Management Journals

Ronald

[email protected]

1-702-714-7001Extn: 9042

Chemistry Journals

Gabriel Shaw

[email protected]

1-702-714-7001Extn: 9040

Clinical Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Engineering Journals

James Franklin

[email protected]

1-702-714-7001Extn: 9042

Food & Nutrition Journals

Katie Wilson

[email protected]

1-702-714-7001Extn: 9042

General Science

Andrea Jason

[email protected]

1-702-714-7001Extn: 9043

Genetics & Molecular Biology Journals

Anna Melissa

[email protected]

1-702-714-7001Extn: 9006

Immunology & Microbiology Journals

David Gorantl

[email protected]

1-702-714-7001Extn: 9014

Materials Science Journals

Rachle Green

[email protected]

1-702-714-7001Extn: 9039

Nursing & Health Care Journals

Stephanie Skinner

[email protected]

1-702-714-7001Extn: 9039

Medical Journals

Nimmi Anna

[email protected]

1-702-714-7001Extn: 9038

Neuroscience & Psychology Journals

Nathan T

[email protected]

1-702-714-7001Extn: 9041

Pharmaceutical Sciences Journals

Ann Jose

[email protected]

1-702-714-7001Extn: 9007

Social & Political Science Journals

Steve Harry

streamtajm

[email protected]

1-702-714-7001Extn: 9042

 
© 2008- 2018 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version