alexa
Reach Us +1-504-608-2390
Hyperglycemia Changes Expression Of Photoreceptors In The Eye | 77436
ISSN: 2155-9570

Journal of Clinical & Experimental Ophthalmology
Open Access

Like us on:

OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations
700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

2nd International Conference on Ophthalmology

Suneeta Yadav
Central University of Jharkhand, India
ScientificTracks Abstracts: J Clin Exp Ophthalmol
DOI: 10.4172/2155-9570-C1-069
Abstract
Zebrafish (Danio rerio) is a good research model to study visual system in vertebrates. In the visual system of Zebrafish, retina is most important part for photoinduction as it has photoreceptor cells called cone cell having photoreceptor protein molecules called opsins, photo-sensitive proteins. A few reports on retinopathy are available on Zebrafish but reports on change of photoreceptors in response to hyperglycemia were rare. To investigate the effect of hyperglycemia on the expression of opsins in the eye of the Zebrafish, adults were divided into two groups: Experimental and control. The experimental group was induced for diabetic condition or hyperglycemia frequently or alternately immersing them in glucose solution and water. Another group i.e., control received normal water and not provided with glucose. At the termination of study i.e., after 30 days of first day treatment, animals of both the groups were sacrificed and blood (for glucose level and stress hormone level), brain (for study of neurons and neuropathy in different areas), kidney (for nephropathy) and eye (for retinopathy and to measure expression of photoreceptors-opsins) were removed. Blood glucose level was measured using glucometer in both the groups and it was observed that glucose level in experimental animals were higher as compare to control. For visual histopathology, eyes were fixed, paraffinized, sectioned, deparaffinized and stained. When histological sections were photographed, visual deteriorations were detected. For immunohistochemistry, deparaffinized sections were rinsed in phosphate buffer saline (PBS) and primary as well secondary antibodies were applied on section to measure immune-reactivity of opsin and its expression. It was observed after photography of immuno-stained sections that immune-reactivity of opsins in experimental or diabetic group was higher as compare to control. These findings suggest that diabetes affects visual system and causes retinopathy. Neuropathy, nephropathy, endocrine disorders and stress study still needs to be studied.
Biography

Suneeta Yadav has completed her PhD from Banaras Hindu University, India and has worked on interaction of photoperiod and temporal phase relation of circadian neural oscillations in the reproductive regulation of Japanese quail. Her area of research was photobiology, reproductive endocrinology and chronobiology. Presently, she is Assistant Professor of Central University of Jharkhand, India. She is also engaged in research on Zebrafish for the study of diabetic retinopathy, neuropathy, nephropathy and hormonal disorders. She has 5 publications in different reputed, peer-reviewed journals and 13 abstracts published in different conferences and symposia with 13 oral/poster/invited presentations accordingly.

image PDF   |   image HTML
 
Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2018-19
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

Agri and Aquaculture Journals

Dr. Krish

[email protected]

+1-702-714-7001Extn: 9040

Biochemistry Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Business & Management Journals

Ronald

[email protected]

1-702-714-7001Extn: 9042

Chemistry Journals

Gabriel Shaw

[email protected]

1-702-714-7001Extn: 9040

Clinical Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Engineering Journals

James Franklin

[email protected]

1-702-714-7001Extn: 9042

Food & Nutrition Journals

Katie Wilson

[email protected]

1-702-714-7001Extn: 9042

General Science

Andrea Jason

[email protected]

1-702-714-7001Extn: 9043

Genetics & Molecular Biology Journals

Anna Melissa

[email protected]

1-702-714-7001Extn: 9006

Immunology & Microbiology Journals

David Gorantl

[email protected]

1-702-714-7001Extn: 9014

Materials Science Journals

Rachle Green

[email protected]

1-702-714-7001Extn: 9039

Nursing & Health Care Journals

Stephanie Skinner

[email protected]

1-702-714-7001Extn: 9039

Medical Journals

Nimmi Anna

[email protected]

1-702-714-7001Extn: 9038

Neuroscience & Psychology Journals

Nathan T

[email protected]

1-702-714-7001Extn: 9041

Pharmaceutical Sciences Journals

Ann Jose

[email protected]

1-702-714-7001Extn: 9007

Social & Political Science Journals

Steve Harry

streamtajm

[email protected]

1-702-714-7001Extn: 9042

 
© 2008- 2018 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version