Hypoxia Increase The Function Of Adipose Tissue-mesenchymal Stem Cells (AT-MSCs)-derived Extracellular Vesicles (EVs) In Wound Healing | 94780
Journal of Tissue Science & Engineering
Like us on:
Our Group organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.
Mesenchymal stem cells (MSCs) ameliorate the impaired wound healing by secreting cytokines, and small particles included
extracellular vesicles (EVs). EVs are fragments membrane containing of nucleic acids and proteins which can be transferred
to the target cells. Recent studies reported MSC-derived EVs (MSC-EVs) had potency to enhance the recovery process of injuries as
the parental cells; therefore EVs-based therapeutics become attractive strategy in clinical application to avoid the immunorejection.
In addition, it is reported that wound healing ability of MSCs is induced by hypoxic pretreatment. However, up to now, the effect
of hypoxia on MSC-EVs for wound healing function had not been clarified yet. Therefore, we analyzed the effects of hypoxia
on adipose tissue-MSCs (AT-MSCs) derived EVs by comparing the characteristics and wound healing functions of EVs under
normoxic (20% O2) or hypoxic (1% O2) condition. Results showed hypoxia induced expression of wound healing-related genes
in EVs. Compared to normoxia-treated EVs, hypoxia-treated EVs showed higher ability to support the wound healing functions
in recipient cells, including MSCs and endothelial progenitor cells (EPCs). MSCs or EPCs co-cultured with hypoxia-treated EVs
showed increased migration ability and upregulation of CXCR4, SDF-1, and VEGF expression. Of note, transplantation of hypoxiatreated
EVs significantly reduced the wound area in diabetic mice compared to mice with normoxia-treated EVs. In conclusion, our
study revealed that hypoxia enhanced the wound healing ability of EVs through paracrine effects on MSCs and EPCs. Therefore,
hypoxia-treated EVs can be considered as a candidate for delayed wound healing of various metabolic diseases.
Peer Reviewed Journals
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals