alexa Identification, Activation And Engineering Production Of The Antitumor UCS1025A | 62793

11th World Congress on Pharmaceutical Sciences and Innovations in Pharma Industry
February 27-28, 2017 Amsterdam, Netherlands

Sameh Soliman
University of Sharjah, UAE
University of California, USA
Posters & Accepted Abstracts: Pharmacoeconomics
DOI: 10.4172/2472-1042.C1.003
Abstract
UCS1025A is a novel polyketide-non-ribosomal peptide (PK-NRP) hybrid fungal metabolite with a decalin-like structure. UCS1025A isolated from Acremonium fungus KY4917 (FERM) and exhibiting antibacterial and antiproliferative activities. Although it is known for a decade, its biosynthesis has not been elucidated. The gene cluster for USC1025A was predicted by bioinformatics analysis and its PKS-NRPS deletion mutation validates its essential role in UCS1025A biosynthesis. Heterogonous engineering of the gene cluster into Aspergillus nidulans using yeast recombination-based cloning strategy was successfully performed. Only the gene cluster transformed without transcription factors (AN#2) was able to produce the compound. Activation of the UCS1025A gene cluster in the FERM fungus by over-expressing each transcription factor separately increased the compound production up to 4 times. Similarly activation of the gene cluster in A. nidulans transformed with the UCS1025A gene cluster containing transcription factors (AN#1) was able to detect affordable amount. Two transcription factors would work as activator and one as repressor. The UCS1025A gene cluster (1759) with another gene cluster (1590) comprised a branch of a phylogenetic tree close to lovastatin gene cluster indicating similar compound could be produced by this cluster. This work will help in genetic manipulation and investigation of the UCS1025A biosynthetic pathway.
Biography

Email: [email protected]

image PDF   |   image HTML
 

Relevant Topics

Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2018-19
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

Agri & Aquaculture Journals

Dr. Krish

[email protected]

1-702-714-7001Extn: 9040

Biochemistry Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Business & Management Journals

Ronald

[email protected]

1-702-714-7001Extn: 9042

Chemistry Journals

Gabriel Shaw

[email protected]

1-702-714-7001Extn: 9040

Clinical Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Engineering Journals

James Franklin

[email protected]

1-702-714-7001Extn: 9042

Food & Nutrition Journals

Katie Wilson

[email protected]

1-702-714-7001Extn: 9042

General Science

Andrea Jason

[email protected]

1-702-714-7001Extn: 9043

Genetics & Molecular Biology Journals

Anna Melissa

[email protected]

1-702-714-7001Extn: 9006

Immunology & Microbiology Journals

David Gorantl

[email protected]

1-702-714-7001Extn: 9014

Materials Science Journals

Rachle Green

[email protected]

1-702-714-7001Extn: 9039

Nursing & Health Care Journals

Stephanie Skinner

[email protected]

1-702-714-7001Extn: 9039

Medical Journals

Nimmi Anna

[email protected]

1-702-714-7001Extn: 9038

Neuroscience & Psychology Journals

Nathan T

[email protected]

1-702-714-7001Extn: 9041

Pharmaceutical Sciences Journals

Ann Jose

[email protected]

1-702-714-7001Extn: 9007

Social & Political Science Journals

Steve Harry

[email protected]

1-702-714-7001Extn: 9042

 
© 2008- 2018 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version