alexa Identification Of Corn Maize Borne Pathogen And Aflatoxins Quantification | 70149
ISSN: 2155-9821

Journal of Bioprocessing & Biotechniques
Open Access

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15th Asia-Pacific Biotechnology Congress

Ndayambaje Jean Bernard
University of Rwanda, Rwanda
Posters & Accepted Abstracts: J Bioprocess Biotech
DOI: 10.4172/2155-9821-C1-013
Abstract
Mycotoxins are toxins produced by organisms categorized as fungi that can be found virtually everywhere and their toxicity occurs at very low concentrations, so sensitive and reliable methods for their detection are required in grains pre-harvest in the field and postharvest in storage. Aflatoxins are a group of mycotoxins produced by Aspergillus species and the effects in animals of ingesting excessive amounts of the toxin range from chronic health and performance problems to death. Fungi grow rapidly when the air has a high relative humidity and slowly at a low relative humidity. In this study, the samples of maize were randomly collected in three sectors: Nyamata, Gakenke and Mahembe Sectors. Using selective media and biochemical tests, the three strains; A. flavus capable of producing both B- and G-type aflatoxin and A. pseudotamarii which has not previously detected in our region and A. parasiticus were isolated from the collected maize and the total aflatoxin (AFT) in maize flour was evaluated by Reveal Q+; the mean concentrations of AFT were 52.3±3.93 CFU/ml for Mahembe sector, 49.5±0.98 CFU/ml for Gakenke sector and 43.7±2.15 CFU/ml for Nyamata sector. The level of AFT contamination in Nyamata, Gakenke and Mahembe sectors was higher than the maximum tolerable limit of aflatoxins recommended by the United States Food and Drug Administration guidelines for aflatoxin in animal feeds and human foods at 20 parts per billion (20 ppb).
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