IL-10 Polymorphism And IL-10 Cytokine Production In Response To Mite Stimuli And Its Association With Atopy And Asthma In Children Living In A Poor Area In Latin America | 67449
Journal of Clinical & Cellular Immunology
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IL-10 polymorphism and IL-10 cytokine production in response to mite stimuli and its association with atopy and asthma in children living in a poor area in Latin America
8th European Immunology Conference
Flavia de Araujo Sena, Alana Alcantara Galvao, Emilia M M de Andrade Belitardo, Gustavo Nunes de Oliveira Costa, Mariese Conceicao Alves dos Santos,
Camila Alexandrina Viana de Figueiredo, Maurício Lima Barreto and Neuza M Alcantara-Neves
Federal University of Bahia (UFBA), Brazil
Rural Federal University of Pernambuco, Brazil
FIOCRUZ – Bahia, Brazil
Rationale: IL-10 is an important regulatory cytokine with a protective role in allergies. This study aim to verify if IL-10 gene
polymorphisms interferes in IL-10 cytokine production according to mite stimuli and atopy/asthma status.
Methods: 1119 subjects from Salvador, Brazil, were genotyped using 2.5 Human Omni Beadchip from Illumina. IL-10 production by
whole blood culture with the following mite stimuli: Blomia tropicalis and Dermatophagoides pteronyssinus was measured by ELISA.
Asthma status was defined by ISAAC questioner. Atopy was determined through skin prick test to regional aeroallergens and specific
IgE levels to B. tropicalis, D. pteronyssinus, Blatella germanica and Periplaneta americana. Statistical analyses were done using PLINK
1.9 and SPSS 22.1.
Results: B. tropicalis was the mite with the higher frequency of sensitized individuals (34.26%) and the bigger frequency of IL-
10 responders (93.1% against 21.3% to D. pteronyssinus). There was no association between asthma severity and B. tropicalis IL-
10 induction. SPT ≥ 3mm for B. tropicalis had a positive correlation with IL-10 production in response to this stimulus (r=0.126,
p=0.031). None genetic variant was associated with IL-10 production by B. tropicalis, furthermore one variant rs3024496 (C allele)
was associated with greater skin reactivity (OR 1.33; 95% CI, 1.02-1.73).
Conclusion: The absence of association between IL-10 polymorphism and this cytokine production can be explained by the lack
of the promoter region of the gene on these analyses. Our findings did not support the IL-10 regulatory role once we did not find
differences between IL-10 cytokine productions according to atopy/asthma status; we otherwise found a positive correlation between
IL-10 production and SPT positivity and a high frequency of IL-10 producers in B. tropicalis stimulus response. Now, we question
what IL-10 function in allergy caused by B. tropicalis, once this mite stimulus IL-10 production and there is association with more
Flávia de Araújo Sena, is graduated in Biomedicine from Bahiana School of Medicine and Public Health. Master in Immunology by the Postgraduate Program in Immunology (PPGIM) at the Federal University of Bahia (UFBA). Currently does a specialization in Microbiology and is a PhD student of Immunology at UFBA, working in the Laboratory of Allergy and Acarology (LAA). She collaborates with the following projects: An asthma cohort in children and adolescents of the city of Salvador – Bahia, SCAALA (2013), Immuno-intervention in experimental models of respiratory allergy and Chagas disease and study of the in vitro immunomodulatory effect of candidin fractions (2015).