Journal of Clinical and Cellular Immunology
Open Access

Immune mechanism as the mechanism maintenance stability of both internal energy an organism as well as internal energy of cells of an organism

3^rd International Conference and Exhibition on Clinical & Cellular Immunology

September 29-October 01, 2014 DoubleTree by Hilton Baltimore-BWI Airport, USA

Michail Ponizovskiy

Accepted Abstracts: J Clin Cell Immunol

Abstract :

During angiogenesis, interaction of endothelial cells (EC) with cells of microenvironment, including monocytes/ macrophages, and the extracellular matrix are controlled by cytokines. The aim of the research was investigation of cytokine influence on capillary-like tube formation of endothelial cells EA.Hy926 in presence of THP-1 cells. For cell activation the following recombinant cytokines were used: bFGF - (BD, США) (1 ng/ml, 10 ng/ml, 20 ng/ml); VEGF - (BD, США) (1 ng/ ml, 10 ng/ml, 100 ng/ml); PlGF - (BD, США) (1 ng/ml, 5 ng/ml, 20 ng/ml); TGFβ - (BD, США) (1 ng/ml, 5 ng/ml, 10 ng/ml). Endothelial cells were seeded on Matrigel-coated 24-well plates (BD, USA) at a density of 150000 cells/well, also were added cytokines and 2,5% fetal bovine serum. To part of wells THP-1 cells were added (250000 cells/well), to part of wells - the cultural medium without THP-1 cells. Capillary tube formation was assessed 24 hours later using microscope AxioObserver Z1. Stimulating effect of bFGF and VEGF on capillary-like tube formation in monoculture and co-culture system was shown, while the effects of cytokines were more pronounced in co-culture system. PlGF had no effect on EC tube formation in monoculture, but at high concentrations proangiogenic activity of PlGF in co-culture system was shown. TGFβ inhibited EC tube formation, while its anti-angiogenic potential was more pronounced in the co-culture system. Thus, cytokines of microenvironment could make a definite contribution to the interaction of EC and monocytes/macrophages during angiogenesis.