Journal of Proteomics & Bioinformatics
Open Access
ISSN: 0974-276X
+44 1223 790975
ISSN: 0974-276X
+44 1223 790975
Xiaoying Zhang
Northwest Agriculture and Forestry University, China
Scientific Tracks Abstracts: J Proteomics Bioinform
Residues of antibiotics in the aquatic environment and in food of animal origin represent a major concern, as prolonged exposure to antibiotics is a serious health hazard, related to both the side effects of prolonged use and the risk of developing bacterial resistance to various antibiotics. Given the low levels of the antibiotics residues in complex matrices, the development of sensitive analytical methods represents a major challenge. We report the current state of the art in the determination of antibiotics based antibody engineering and immunoassays. First, diversified antibody generation platforms/methods promote the screening and assessment of high qualified antibodies. Second, various immunoassays simplify and empower the immune-detection applications for different biomedical purposes. Third, there are tremendous academic motivations and industrial needs in the detection/evaluation of antibiotics by using antibodies and immunoassays. There are diversified antibody generation platforms/methods (e.g., Polyclonal antibody (pAb), Cell engineering antibodies��?hybridoma technology, recombinant antibdy (rAb)). Different species of antibodies e.g. chicken antibody (polyclonal IgY, monoclonal IgY), camel antibodies, and rabbit antibodies have been widely used. The recent advancements in antibody engineering permit to produce chicken mIgY quickly and inexpensively by phage display technology, because chicken possess only one functional immunoglobulin heavy chain variable region (VH) gene and one light chain variable region (VL) gene. The purpose of our study is to prove that the mIgY could be used for immunological detection and diagnosis, for screening and validating of biomarkers and drug targets. There is a huge demand for the detection reagent of antibiotic residues on the market, and it gradually becomes the intrinsic driving force of the development of industry. Diversified antibody generation platforms/methods promote the screening and assessment of high qualified antibodies. Various immunoassays simplify and empower the immune-detection applications for different biomedical purposes.
Xiaoying Zhang is Professor at Northwest Agricultural and Forestry University (elite university in the national 985 and 211 programs), China since 2008. He is graduated from China Pharmaceutical University (undergraduate study in Pharmacology) and Charité Medical School (Post-graduate and PhD in Pharmacology) of Freie Universität Berlin and Humboldt-Universität Zu Berlin, Germany. Before and during his PhD study, he has worked in WHO- headquarters Geneva and Robert- Koch Institute Berlin. He was the nominee for the outstanding Young Scientist Award, China (2015). His major research interests are: antibody engineering, immunoassay and rapid detection/ diagnosis, nature product research and pharmacological study. He has contributed 100+ publications (from which 80+ international publications). He is holding three Chinese patents for his innovative research accomplishments. He has also edited five books (four– Chinese and one in English) and contributed three book chapters in the books of International publishers. He has been appointed as editor and/or reviewer in more than 40 international journals and serves as Member of Board of Examiner in universities from China, Germany, India and Pakistan.