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An immunomagnetic separation (IMS) method was developed for separating Salmonella bacteria from large-volume
samples of raw eggs.An egg was homogenized with a blender.The homogenized egg material was diluted with a 0.05%
Triton X-100 solution to make a 200 mL sample mixture.Anti-Salmonella typhimurium antibody coated magnetic micro
particles (MMP) were used to trap Salmonellatyphimurium bacteria in the sample mixture.The Salmonella-trapped MMP were
separated from the egg sample mixture by using a large magnet.An enzyme-linked immunosorbent assay method was adopted
and revised for detecting the bacteria trapped onto the MMP.Horseradish peroxidase labeled anti-Salmonellatyphimurium
antibody was used to label the trapped bacteria, and a SureblueTM solution was used as a substrate.The color compound
resulted from horseradish peroxidase catalyzed reaction was detected with UV/Vis absorption spectrometry using a 1 cm
sample cell.This simple method can detect 1.4x107Salmonellatyphimurium cells in one raw egg (7.0*104 Salmonella cells/
mL in a sample mixture) without any pre-enrichment.The method is quick, can obtain test results within 5 hours. The results
presented in this poster demonstrate the feasibility of using IMS for separating bacteria from large volume complex samples,
which could be adopted for detecting bacteria in other type samples in food safety inspection.
Shiquan Tao completed his PhD in Chemistry from Hiroshima University, Japan. He is an Associate Professor of Chemistry at West Texas A&M University with
research interest in the development of fiber optic chemical/biochemical sensors for monitoring industrial processes, environmental monitoring as well as for quick
detecting foodborne pathogens. Before joining the faculty at WTAMU, he was a research faculty at the Diagnostic Instrumentation and Analysis Laboratory of
Mississippi State University in charge of the institute?s fiber optic sensor research program for US DOE? Office of Science?s Environmental Management Program.
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