alexa
Reach Us +1-947-333-4405
Immunomodulatory Functions Of Defensins, Innate Molecules, As Mucosal Adjuvant With The Peptide Antigens Of HIV-1 At The Mucosal Surfaces | 9846
ISSN: 2157-7560

Journal of Vaccines & Vaccination
Open Access

Like us on:

OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations
700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

3rd International Conference on Vaccines & Vaccination

Teena Mohan and D. N. Rao
Young Research Forum: J Vaccines Vaccin
DOI: 10.4172/2157-7560.S1.016
Abstract
Introduction: The mechanisms of resistance to HIV infection in the human oral cavity are incompletely understood while salivary components have been implicated in protection. There are growing evidences that human defensin peptides originating in the oral epithelial cells may be playing an important role in the prevention of HIV infection. Methodology: We have synthesized HIV and Defensin peptides and their corresponding analogues by making some modifications in the natural sequence. We have done Anti HIV, Anti-microbial and other characteristic study of defensins to prove them active. Then, immunized theses formulations in Outbred and Two different Inbred mice (H 2b , H 2d ) through IN route using Microsphere as delivery vehicle. We have studied Humoral Response of HIV peptides with and without Defensins by estimating antibody levels (IgG/IgA) in the serum as well as in lung, intestinal, vaginal and rectal washes till day 120. For cell mediated immune response, peptide specific T cell proliferation and cytokine/chemokine levels were studied in the cells isolated from the three different mucosal sites i.e. spleen, lamina propria and peyer?s patches of the primed mice. Simultaneously, we have done Cytolytic activity analysis, by estimating IFN-γ/Perforin secretion by CD4+ also through FACS, which was checked by IFN- γ/Perforin secretion. Result & Discussion: The HIV peptides alone in microsphere showed low peptide specific response of peak titre in sera and different washes while the presence of defensins increased significantly this titre both in sera (1,02,400-4,09,600) as well as in washes (800-12,800) (p<0.05) Very interestingly, we have found that the cellular immunogenicity of all the HIV peptides with defensin peptides in different formulations showed a significantly higher (upto 2 fold ranging from 10-50 stimulation index) (p<0.001) proliferation response as compared to HIV peptide alone. The cytokine measurement profile showed mixed Th1 and Th2 type of immune response. The FACS analysis data revealed that CD8 + / CD4 + T cells showed significantly higher Cytolytic activity in the HIV with Defensin peptide formulations. Surprisingly, CD4 + T-cells were also showing Cytolytic property. Conclusion: We have shown from the present study that these defensin peptides and their analogues are markedly enhanced the antigen specific immune response even at very low concentration. Thus, the results reported here demonstrate the effectiveness of synthetic defensin peptide analogues to induce strong and long lasting humoral and cellular immune response through intranasal route using PLG- microsphere as a delivery vehicle. Our findings may have implications in the development of new antiviral agent for AIDS therapy.
image PDF   |   image HTML
 
Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2018-19
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

Agri and Aquaculture Journals

Dr. Krish

[email protected]

+1-702-714-7001Extn: 9040

Biochemistry Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Business & Management Journals

Ronald

[email protected]

1-702-714-7001Extn: 9042

Chemistry Journals

Gabriel Shaw

[email protected]

1-702-714-7001Extn: 9040

Clinical Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Engineering Journals

James Franklin

[email protected]

1-702-714-7001Extn: 9042

Food & Nutrition Journals

Katie Wilson

[email protected]

1-702-714-7001Extn: 9042

General Science

Andrea Jason

[email protected]

1-702-714-7001Extn: 9043

Genetics & Molecular Biology Journals

Anna Melissa

[email protected]

1-702-714-7001Extn: 9006

Immunology & Microbiology Journals

David Gorantl

[email protected]

1-702-714-7001Extn: 9014

Materials Science Journals

Rachle Green

[email protected]

1-702-714-7001Extn: 9039

Nursing & Health Care Journals

Stephanie Skinner

[email protected]

1-702-714-7001Extn: 9039

Medical Journals

Nimmi Anna

[email protected]

1-702-714-7001Extn: 9038

Neuroscience & Psychology Journals

Nathan T

[email protected]

1-702-714-7001Extn: 9041

Pharmaceutical Sciences Journals

Ann Jose

[email protected]

1-702-714-7001Extn: 9007

Social & Political Science Journals

Steve Harry

[email protected]

1-702-714-7001Extn: 9042

 
© 2008- 2018 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version