alexa Impact Of Cold Atmospheric Pressure Plasma On Human Cells | 13736
ISSN: 2161-1009

Biochemistry & Analytical Biochemistry
Open Access

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International Conference and Exhibition on Biochemical & Molecular Engineering

Kai Masur, Sybille Hasse, Kristian Wende, Anke Schmidt, Annemarie Barton, Sander Bekeschus, Lena Bundscherer, Ulrike Lindequist,Axel Kramer and Klaus-Dieter Weltmann
ScientificTracks Abstracts: Biochem Anal Biochem
DOI: 10.4172/2161-1009.S1.002
Abstract
Physical cold atmospheric pressure plasma consists of components such as charged particles, reactive oxygen and nitrogen species (ROS/RNS) including bioactive substances (e.g. OH, NO) as well as radiation (ranging from IR to UV), and free electrons [1]. Due to recent advances in the development of non-thermal plasmas sources, the treatment of living matter with a blend of various plasma components became possible opening numerous possibilities for plasma to influence cells on a molecular/ genetic level. Cold plasmas has recently been shown to have broad application potential and therefore promise improvement in treating infected or chronic wounds, superficially skin infections and other demanding skin diseases. While lots of data exists about the killing of microorganisms as well as mammalian cells, the plasma-mediated activation of human cells still remains to be investigated. The aim of this work was to analyze plasma-mediated activation of human cells of different origin (skin, connective tissue and immune system). Therefore human keratinocytes (HaCaT), fibroblasts (MRC5) and immune cells (Jurkat T-cells and THP1 monocytes) were investigated by genomic and proteomic approaches. Investigating the cellular responses to non-thermal plasma treatment, we were able to identify several cell specific genes and proteins, which were activated after plasma treatment[2]. Especially cell signaling and pro-proliferative signal molecules were activated after short term plasma treatment indicating stimulatory effects of non-thermal plasmas[3]. However, while all types of cells showed a comparable pattern of activated molecules after plasma treatment, there are some differences in the cellular reactions, displaying diverse sensitivities of the investigated cells towards cold plasma treatment.
Biography

Kai Masur has completed his Ph.D. at the age of 28 years from Witten/Herdecke University and postdoctoral studies from TUFTs University School of Medicine. He is the group leader and principal investigator of the ZIK plasmatis group Cellular Effects, at the Leibniz Institute for Plasma Science and Technology, Greifswald, Germany. He has published more than 30 papers in reputed journals.

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