Type IIdiabetes is a metabolic disease which is tuned by diet. Therefore, it makes sense to explore the different dietary constituents
that may have positive effect on diabetes. Male albino wistar rats were fed on high fat diet (HFD, 35%) for three weeks and
after that injected with streptozotocin (STZ, 35 mg/kg) intraperitoneally. Then after three days, rats having fasting blood glucose
(FBG) >300 mg/dl were divided into groups: HFD+skim milk (HFD+SM); HFD+milk fermented with Lactobacillus rhamnosus
GG (HFD+LGG); HFD+milk fermented with L. rhamnosus NCDC 17 (HFD+17), and fed for another 6 weeks. There was no
loss in body weight due to STZ in HFD+17 group but substantial loss was observed in HFD+SM and HFD+LGG groups, yet
this difference was not statistically significant. HFD+17 group had improved OGTT, lower FBG, GHb, FFAs, triglycerides, total
cholesterol, LDL-cholesterol and atherogenic index, and higher plasma insulin and HDL-cholesterol in comparison to HFD+SM
and HFD+LGG groups. Even though the total number of bacteria was same, HFD+17 had more bifidobacteria and lactobacilli in
caecal contents in comparison to HFD+LGG. Relative propionate proportions (%) in caecal contents were significantly lower in
case of HFD+17 then HFD+SM and HFD+LGG groups. HFD+17 showed increased expression of proglucagon and PC1 genes in
cecum and decreased expression of TNF-α and IL-6 genes in epididymal fat in comparison to HFD+SM and HFD+LGG groups.
We conclude that L. rhamnosus NCDC 17 has anti-diabetic effect in HFD fed and STZ treated rats, and this effect was achieved
by improvement of gut function.
Satvinder Singh has completed his Ph.D. (Animal Biochemistry) in July 2012 at the age of 28 years from National Dairy Research Institute (NDRI),
Karnal, Haryana, India. He is a B.Sc and M.Sc. in Biochemistry from Kurukshetra University, Kurukshetra, Haryana, India. He is currently a
Research Associate in the Dairy Microbiology Division, NDRI. His area of research is on functional food components, prebiotics, probiotics and
synbiotics for gut health, and molecular mechanism of their effects in relation to obesity, Type II diabetes and low grade inflammation.
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