This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)
All submissions of the EM system will be redirected to Online Manuscript Submission System. Authors are requested to submit articles directly to Online Manuscript Submission System of respective journal.
Contemporary understanding of memory formation relies on the concept of long-term synaptic plasticity, for which protein
synthesis is a vital requirement. The best understanding of the synaptic plasticity comes from the studies of hippocampus,
playing the central role in memory formation. Deterioration of hippocampal activity is associated with multiple forms of cognitive
impairments and neurodegeneration. Despite extensive studies to elucidate mechanisms of memory traces formation and identity
of the involved molecular factors is still elusive. Using the advantage of radial arm maze long-term spatial memory paradigm, we
investigated protein turnover in mouse hippocampus during the learning. Utilizing quantitative proteomics, we identified 1592
proteins, exhibiting a complex picture of expression changes during memory formation. Variable linear decomposition enriched
factors responsible for memory-related protein levels’ at: The initial (167 proteins), the steep learning improvement (150 proteins)
and final phases (123 proteins). Gene ontology and signaling pathways analysis revealed differential enrichment of: a) neurotrophic
factors signaling pathways, proteins regulating synaptic transmission, microfilament assembly during the first day of learning
curve; b) transcription and translation machinery, protein trafficking, metabolic activity and Wnt pathway during the steep phase
of learning; c) cytoskeleton organization proteins at the final step. Network analysis of protein expression profiles revealed candidate
key regulators of memory formation. Further, the role of two selected candidates was confirmed in synaptic plasticity. Summarizing,
identification of the key regulators opens new horizons in understanding of memory formation molecular mechanisms, as well as in
therapeutic targeting to shut down pathogenetic pathways of memory impairment.
I Michaelevski has completed his PhD in 2005 at Tel Aviv University and Post-doctoral studies from Weizmann Institute of Science and University of California, San Francisco as a Visitor Post-doctoral Fellow. He is an Independent Investigator from 2011 at Tel Aviv University and now in Ariel University. He is Head of the Molecular and Functional Neurobiology Laboratory.