alexa In Vitro Cell Mobility: A Mesenchymal Stem Cells Marker For Multipotency?
ISSN: 2157-7552

Journal of Tissue Science & Engineering
Open Access

Like us on:
OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations

700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

3rd International Conference on Tissue Science & Regenerative Medicine
September 24-26, 2014 Valencia Convention Centre, Spain

Alessandro Bertolo
Posters: J Tissue Sci Eng
DOI: 10.4172/2157-7552.S1.015
Abstract
M esenchymal stem cells (MSCs) are fundamental players in future cell-based therapies. These cells possess high proliferative ability, multilineage potential and immunomodulatory properties, all requisites essential for cell therapies. Nevertheless, intra-donor variability of MSCs is not fully characterized yet, leading to uncertain outcomes in the use of these cells. Here we propose a life imaging tool to characterize population of human MSCs derived from different donors, based on the in vitro mobility of the cells and to correlate results to the respective differentiation potential. Bone marrow human MSCs from 20 donors and at various in vitro passages (from P3 to P13) were evaluated. Track length of 70 cells per population were measured over a period of 24 hours, and distances were compared with the respective adipogenic, chondrogenic and osteogenic differentiation potential. We found that senescent populations were enriched with slow moving cells, compared with younger ones. Cells with larger cell body were moving less compared to smaller ones, while spindle shaped cells had an average speed. Our preliminary data showed that both fast cells and slow cells were characterized by low differentiation potential, while average moving cells were more effective in undergoing all three lineage differentiation. Heterogeneity in single cell motility within a population correlated with faster moving cells, while populations rich in slow moving cells tended to homogeneity (only senescent cells). In conclusion, in vitro cell mobility might be an useful tool to characterize MSC population and recognize prior to use the differentiation potential of MSCs.
Biography
Alessandro Bertolo has completed his PhD at the age of 32 years old at University of Bern, Switzerland. His PhD focused on the development of tissue engineering approaches based on mesenchymal stem cells application to treat intervertebral disc degeneration. He is currently involved in intervertebral disc studies both at in vitro and in vivo levels, as postdoctoral fellowship. Previously, he worked as fellow at S. Raffaele hospital, Milan, in the field on neural stem cells
image PDF   |   image HTML
 

Relevant Topics

Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

Agri & Aquaculture Journals

Dr. Krish

[email protected]

1-702-714-7001Extn: 9040

Biochemistry Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Business & Management Journals

Ronald

[email protected]

1-702-714-7001Extn: 9042

Chemistry Journals

Gabriel Shaw

[email protected]

1-702-714-7001Extn: 9040

Clinical Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Engineering Journals

James Franklin

[email protected]

1-702-714-7001Extn: 9042

Food & Nutrition Journals

Katie Wilson

[email protected]

1-702-714-7001Extn: 9042

General Science

Andrea Jason

[email protected]

1-702-714-7001Extn: 9043

Genetics & Molecular Biology Journals

Anna Melissa

[email protected]

1-702-714-7001Extn: 9006

Immunology & Microbiology Journals

David Gorantl

[email protected]

1-702-714-7001Extn: 9014

Materials Science Journals

Rachle Green

[email protected]

1-702-714-7001Extn: 9039

Nursing & Health Care Journals

Stephanie Skinner

[email protected]

1-702-714-7001Extn: 9039

Medical Journals

Nimmi Anna

[email protected]

1-702-714-7001Extn: 9038

Neuroscience & Psychology Journals

Nathan T

[email protected]

1-702-714-7001Extn: 9041

Pharmaceutical Sciences Journals

Ann Jose

[email protected]

1-702-714-7001Extn: 9007

Social & Political Science Journals

Steve Harry

[email protected]

1-702-714-7001Extn: 9042

 
© 2008- 2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version