alexa In Vitro Monitoring Of Uric Acid Excretion With Different Diets In Human Urine During 24 H By Flow Injection Chemiluminescence
ISSN: 0975-0851

Journal of Bioequivalence & Bioavailability
Open Access

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5th World Congress on Bioavailability and Bioequivalence Pharmaceutical R&D Summit
September 29-October 01, 2014 DoubleTree by Hilton Baltimore-BWI Airport, USA

Jiajia Wang and Zhenghua Song
Accepted Abstracts: J Bioequiv Availab
DOI: 10.4172/0975-0851.S1.019
Abstract
A sensitive method for determining uric acid (UA) at nanogram level by flow injection (FI) chemiluminescence (CL) was described, based on the inhibitory effect of UA on luminol-KIO4 CL system. It was found that the CL intensity decrements were linearly proportional to the logarithm of UA concentrations ranging from 3.0 to 500.0 ng mL−1 with the detection limit of 1.0 ng mL−1 (3σ). At a flow rate of 2.0 mL min−1, the whole determining performance including sampling and washing could be accomplished in 36.0 s, offering the sample efficiency of 100 h−1. The proposed method was successfully applied to in vitro monitoring of UA excretion in human 24 h urine with different diets, with results showing that porridge, vegetables and meats intake caused differential UA excretions of 722, 767 and 854 mg, respectively
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