alexa In Vivo Tissue Engineered Blood Vessels | 62642
ISSN: 2329-6925

Journal of Vascular Medicine & Surgery
Open Access

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World Congress on Vascular Diseases, Medicine & Surgeons Summit
October 24-25, 2016 Chicago, USA

J I Rotmans
Leiden University Medical Center, The Netherlands
Posters & Accepted Abstracts: Vasc Med Surg
DOI: 10.4172/2329-6925.C1.003
Abstract
There's a large clinical need for novel vascular grafts. Tissue engineered blood vessels (TEBVs) have great potential to improve the outcome of vascular grafting procedures. We present a novel approach to generate autologous TEBV in vivo. Polymer rods were engineered and implanted, evoking an inflammatory response that culminates in encapsulation by a fibrocellular capsule. We hypothesized that, after extrusion of the rod, the fibro-cellular capsule differentiates into an adequate vascular conduit once grafted into the vasculature. Rods were implanted subcutaneously in pigs. After 4 weeks, rods with tissue capsules grown around it were harvested. Tissue capsules were grafted bilaterally as carotid artery interposition. One and 4-week patency were evaluated by angiography where upon pigs were sacrificed. Tissue capsules before and after grafting were evaluated on tissue remodeling using immunohistochemistry, RNA profiling and mechanical testing. Rods were encapsulated by thick, well-vascularized tissue capsules, composed of circumferentially aligned fibroblasts, collagen and few leukocytes, with adequate mechanical strength. Patency was 100% after 1 week and 87.5% after 4 weeks. After grafting, tissue capsules remodeled towards a vascular phenotype. Gene profiles of TEBVs gained more similarity with carotid artery. Wall thickness and aSMA-positive area significantly increased. Interestingly, a substantial portion of (myo) fibroblasts present before grafting expressed smooth muscle cell markers. While leukocytes were hardly present anymore, the lumen was largely covered with endothelial cells. Burst pressure remained stable after grafting. In conclusion, autologous TEBVs can be created in the subcutis, with sufficient mechanical strength enabling vascular grafting. These grafts differentiate towards a vascular phenotype upon grafting.
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