alexa Inducing The Immune Responses By Two Novel Plasmodium Vivax Circumsporozoite Based Vaccine Candidates Formulated In A New Adjuvant System In C57BL/6 Mice | 68584
ISSN: 2155-9597

Journal of Bacteriology & Parasitology
Open Access

Like us on:
OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations

700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

3rd World Conference on Parasitology & Pathogenesis
July 12-13, 2017 Chicago, USA

Samaneh H Shabani, Sedigheh Zakeri, Ali H Salmanian, Jafar Amani, Akram A Mehrizi, Georges Snounou, Yousef Mortazavi and Navid D Djadid
Pasteur Institute of Iran, Iran
Zanjan University of Medical Sciences, Iran
National Institute of Genetic Engineering and Biotechnology, Iran
Baqiyatallah University of Medical Sciences, Iran
Pasteur Institute, France
Posters & Accepted Abstracts: J Bacteriol Parasitol
DOI: 10.4172/2155-9597-C1-032
Abstract
Statement of the Problem: The majority of malaria vaccine studies have mainly focused on Plasmodium falciparum, but Plasmodium vivax have been ignored. However, P. vivax is able to develop hypnozoites, which increases the morbidity caused by a single infection and sever malaria. Additionally, a vaccine targeting of P. vivax represents a necessary tool when considering the elimination/ eradication goal. Many efforts have been conducted to design a vaccine based on circumsporozoite protein (CSP). Because of limited immunogenicity and less effectiveness of protein-based vaccines, these types of vaccines require adjuvant to induce a protective and long-lasting immune response. In this investigation, we evaluate the immunological potency of two newly designed PvCSP based vaccines in combination with a novel adjuvant system (AS). Methodology & Theoretical Orientation: Both CS127 and CS712 constructs include N- and C-terminal parts and a truncated region containing repeat sequences with different arrangement from both PvCSVK210 and PvCSVK247 subtypes. After expression, purification, desalt and concentration, constructs were formulated with novel AS (NLX, CpG and QS21). 6-8 weeks female C57BL/6 mice were immunized with 3 boosts with 2 week interval. Humoral responses include specific antibodies and subclasses against PvCSP, titration and avidity of antibodies, and cellular responses includes lymphocyte proliferation assay and cytokine profiles were evaluated with ELISA, MTT and cytokine assay, respectively. Findings: Our results show that both constructs are highly immunogenic in C57BL/6 mice. Both candidates in combination with AS induce high levels of antibody against PvCSP with high titration and avidity of Th1 related antibodies. Analysis of the induced T-cells highlighted different cytokine profile with significant secretion of IFN-γ and TH1 responses. Conclusion & Significance: Further clinical investigation is needed of these two candidates in primate models to reach the added value in both immunogenicity and protective efficacy.
Biography

Email: [email protected] [email protected]

image PDF   |   image HTML
 

Relevant Topics

Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2018-19
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

Agri & Aquaculture Journals

Dr. Krish

[email protected]

1-702-714-7001Extn: 9040

Biochemistry Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Business & Management Journals

Ronald

[email protected]

1-702-714-7001Extn: 9042

Chemistry Journals

Gabriel Shaw

[email protected]

1-702-714-7001Extn: 9040

Clinical Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Engineering Journals

James Franklin

[email protected]

1-702-714-7001Extn: 9042

Food & Nutrition Journals

Katie Wilson

[email protected]

1-702-714-7001Extn: 9042

General Science

Andrea Jason

[email protected]

1-702-714-7001Extn: 9043

Genetics & Molecular Biology Journals

Anna Melissa

[email protected]

1-702-714-7001Extn: 9006

Immunology & Microbiology Journals

David Gorantl

[email protected]

1-702-714-7001Extn: 9014

Materials Science Journals

Rachle Green

[email protected]

1-702-714-7001Extn: 9039

Nursing & Health Care Journals

Stephanie Skinner

[email protected]

1-702-714-7001Extn: 9039

Medical Journals

Nimmi Anna

[email protected]

1-702-714-7001Extn: 9038

Neuroscience & Psychology Journals

Nathan T

[email protected]

1-702-714-7001Extn: 9041

Pharmaceutical Sciences Journals

Ann Jose

[email protected]

1-702-714-7001Extn: 9007

Social & Political Science Journals

Steve Harry

[email protected]

1-702-714-7001Extn: 9042

 
© 2008- 2018 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version