Inflammatory cytokine profile in patients with diabetic foot infections

Shalbha Tiwari, Daliparthy Devi Pratyush and Surya Kumar Singh

: J Bioengineer & Biomedical Sci

Abstract :

Diabetic foot infection represents a physiological state where the role of inflammatory cytokine is very crucial. They are not only involved in pathogenesis of diabetes but are required for defense against infectious microorganisms. The study was aimed to compare the level of IL6, IL1 and TNF-α in diabetic patients with and without foot infection and to evaluate the influence of individual cytokine on other. A total of 221 (109-diabetic; 112-diabetic foot) cases were enrolled and cytokines (IL1, IL6 & TNF α) were estimated. Group means were compared by independent T test for age, duration of diabetes, HbA1c, BMI and each cytokine and non parametric correlation analysis was performed for each cytokine with one another. There was no significant difference in the age, duration of diabetes, HbA1c and BMI of the two groups. Level of IL1 (101.5±20.8; 49.3±10.8; p=.02) and IL6 (128.3±7.4; 87.0±9.0; p=.001) was significantly higher in diabetic foot than diabetic group but for TNF-α (182.3±20.8; 96.5±22.9; p=.006) the difference could not reach the statistical significance. The correlation analysis showed a significant positive correlation of each cytokine with other in total patients and also in the two groups. Higher level of inflammatory cytokine in diabetic foot indicates that infection modulates the inflammatory response. A significant positive correlation of one cytokine with others shows that release of one cytokine induces the production of other cytokines. There appears some dysregulation of these cytokines, significance of which needs to be elucidated.

Biography :

Shalbha Tiwari (M.Sc Applied Microbiology from Uttar Pradesh, India) and Daliparthy Devi Pratyush (M.Sc Biochemistry from Andhra Pradesh, India) are currently pursuing their Ph.D under supervision of Prof. Surya Kumar Singh, from Department of Endocrinology & Metabolism, Institute of Medical Sciences, Banaras Hindu University, Varanasi-221005. Surya Kumar Singh is Professor in the Department of Endocrinology & Metabolism, IMS, BHU. He has completed his MD & DM (Endo.) from BHU. He has more than 80 publications in reputed journals.