Inhibitory Effect Of Bauhinia Rufa On Oxidative Stress And Enzymes Related To Metabolic Syndrome | 82538
Pharmacoeconomics: Open Access
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Inhibitory effect of Bauhinia rufa on oxidative stress and enzymes related to metabolic syndrome
Joint Event on 12th World Congress on Pharmaceutical Sciences and Innovations in Pharma Industry & 9th Edition of International Conference on Alternative Medicine
Paola dos Santos da Rocha, Jaqueline Ferreira Campos, Vanessa Marina Branco Paula, Valeria Nunes de Souza, Luiza Antas Rabelo, Ana Paula de Araujo Boleti, Maria Leticia Miranda Fernandes Estevinho, Edson Lucas dos Santos and Kely de Picoli Souza
Federal University of Grande Dourados, Brazil
Polytechnic Institute of BraganÃ§a, Portugal
Federal University of Pernambuco, Brazil
Federal University of Alagoas, Av. Lourival Melo Mota, Brazil
The metabolic syndrome is characterized by a set of metabolic risk factors that increase the chance of developing cardiovascular diseases and type 2 diabetes. The present study was carried out to investigate the antioxidant potential and inhibitory of enzymes related to the metabolic syndrome of leaf methanolic extract of Bauhinia rufa (Bong.) Steud. (MEBr). Antioxidant activity was evaluated by DPPH and ABTS free radical scavenging assays, inhibition of Î²-carotene bleaching, FRAP, reducing power, evaluation of the antioxidant enzyme activity SOD, CAT and GPx, reduction of malonyldialdehyde (MDA) in human erythrocytes and inhibition of formation of advanced glycation products (AGEs) via fructose and via glyoxal. Enzymes related to metabolism investigated were Î±-amylase, Î±-glycosidase and lipase. The MEBr (1) presented antioxidant activity in the free radical capture assays DPPH (IC50 8.23Â±0.46 Î¼g/mL), ABTS (IC50 5.24Â±0.37 Î¼g/mL), inhibition of Î²- carotene bleaching (IC50 196.99Â±20.51 Î¼g/mL), FRAP (EC50 125.88Â±4.81 Î¼g/mL) and reducing power (EC50 231.11Â±2.87 Î¼g/mL); (2) stimulated the activity of SOD (16.64Â±1.40%) in the concentrations of 50 to 250 Î¼g/ mL and did not alter the activity of CAT and GPx; (3) reduced the oxidative stress observed by the lower MDA content generated at concentrations of 75 Î¼g/mL (27.95Â±7.58%), 100 Î¼g/mL (50.18Â±2.54%), 125 Î¼g/mL (59.47Â±6.24%), and 250 Î¼g/ mL (72.81Â±2.45%); (4) presented anti-glycation activity by reducing fructose-generated AGEs (IC 50 2.76Â±0.09 Î¼g/ml) and via glyoxal (IC 50 53.13Â±0.43 Î¼g/mL); and (5) inhibited Î±-amylase (IC50 6.80Â±0.09 Î¼g/mL), Î±-glycosidase (IC50 16.54Â±0.86 Î¼g/mL) and lipase activity (IC50 65.98Â±0.55 Î¼g/mL). Taken together, these data reveal the therapeutic potential of MEBr for the prevention and treatment of metabolic disorders.
Paola dos Santos da Rocha is a Biologist and pursuing PhD in Biotechnology and Biodiversity at Federal University of Grande Dourados, MS, Brazil. Her research interests include bioprospecting of medicinal plants and endophytic fungi applied to health bioassays (metabolic syndrome, diabetes, obesity, dyslipidemias, oxidative stress and cancer).
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