alexa Insight Into The Biochemical, Kinetic And Spectroscopic Characterization Of Garlic (Allium Sativum) Phytocystatin: Implication For Cardiovascular Disease
ISSN: 2153-0769

Metabolomics:Open Access
Open Access

Like us on:
OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations

700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

Share This Page

Additional Info

Loading
Loading Please wait..
 

8th International Conference and Exhibition on Metabolomics & Systems Biology
May 08-10, 2017 Singapore

Mohd Faizan Siddiqui, Azaj Ahmed and Bilqees Bano
Aligarh Muslim University, India
Posters & Accepted Abstracts: Metabolomics
DOI: 10.4172/2153-0769-C1-038
Abstract
Phytocystatins are cysteine proteinase inhibitors present in plants. They play crucial role in maintaining protease-anti protease balance and are involved in various endogenous processes. Thus, they are suitable and convenient targets for genetic engineering which makes their isolation and characterization from different sources the need of the hour. In the present study, a phytocystatin has been isolated from garlic (Allium sativum) by a simple two-step process using ammonium sulfate fractionation and gel filtration chromatography on Sephacryl S-100 HR with a fold purification of 152.6 and yield 48.9%. A single band on native gel electrophoresis confirms the homogeneity of the purified inhibitor. The molecular weight of the purified inhibitor was found to be 12.5 kDa as determined by SDS-PAGE and gel filtration chromatography. The garlic phytocystatin was found to be stable under broad range of pH (6-8) and temperature (30-60 °C). Kinetic studies suggest that garlic phytocystatins are reversible and non-competitive inhibitors are having highest affinity for papain followed by ficin and bromelain. UV and fluorescence spectroscopy revealed significant conformational change upon garlic phytocystatin-papain complex formation. Secondary structure analysis was performed using CD and FTIR. Garlic phytocystatin possess 33.9% alpha-helical content as assessed by CD spectroscopy.
image PDF   |   image HTML
 
Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

 
© 2008-2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version
adwords