alexa Integrative Approaches Through Transcriptome Profiling To Identify Subset-specific Gene Activity In Myeloma
ISSN: 2157-7552

Journal of Tissue Science & Engineering
Open Access

Like us on:
OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations

700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

Share This Page

Additional Info

Loading
Loading Please wait..
 

8th International Conference on Tissue Science and Regenerative Medicine
September 11- 12, 2017 Singapore

Siti Sarah Daud, Ryo Takahashi, Nobuyo Yawata, Chng Wee Joo and Makoto Yawata
National University of Singapore, Singapore
Fukuoka Dental College, Japan
ScientificTracks Abstracts: J Tissue Sci Eng
DOI: 10.4172/2157-7552-C1-036
Abstract
Statement of the Problem: The heterogeneous subsets in hematological malignancy such as multiple myeloma may be better characterize when tumor profiling were performed in multiple different dimensions. An integrative approach is needed to predict cell subset with potentially higher clonogenic potential and this has been a long-standing question in myeloma. However when analyzing bone marrow (BM) aspirates for RNA studies, one major challenge is to properly exclude signatures of nonmyelomatous populations from the actual signatures of myeloma subsets that coexist within the same BM niche. Although CD138 is constitutively expressed in aberrant plasma cells, several patients do not express CD138 at high levels. These cases warrant further investigation before they can be subjected for downstream gene expression studies. Additional markers such as CD319 or CD229 were found to be useful since they were highly expressed in myeloma but not in normal plasma cells. Methodology & Theoretical Orientation: The tumor cells identified from primary CD138hi myeloma population were sorted into four subsets using fluorescence-activated cell sorting based on expression of CD19, CD20, CD27 and CD56 surface markers. The sorted cells were subjected to RNA-sequencing and low-input microarray workflows. Findings: The overall proximity between myeloma subsets were assessed using eigengene modules and cluster analyses. For myeloma that lack CD19 surface marker density, several distinct cellular immunophenotypes were identified. Two of the subsets show large similarity in transcription profile. Since they also lack CD27 surface expression, these clones could actually escape apoptosis induced by CD27-CD70 ligand interactions, as compared to the rare CD27hi myeloma cells. Conclusion & Significance: Together, high-dimensional data extracted using combination of clinically relevant markers along with sufficient set of exclusion markers will permit mining for functional differences or similarity between subsets that might not be previously manifested in the bulk primary tumor population.
Biography

Siti Sarah Daud has received her PhD in Leukemia Research from School of Medicine, Cardiff University, UK in 2014. Prior to that, she pursued Masters in Medical Science, focusing on childhood leukemia at University of Malaya, Malaysia. During her postgraduate years, she had served at Pediatric Oncology Research Unit and was involved in chimerism typing for post-hematopoietic stem cell transplant patients at University of Malaya. Presently, she holds a Research Fellow Position at Department of Pediatrics, National University of Singapore. Her current research focuses on understanding the relation of heterogeneity in multiple myeloma subpopulations towards natural killer-cell immune response. She also has experience to ascertain low-input transcriptome workflows for human and non-human primate’s platforms.

image PDF   |   image HTML
 

Relevant Topics

Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

Agri, Food, Aqua and Veterinary Science Journals

Dr. Krish

[email protected]

1-702-714-7001 Extn: 9040

Clinical and Biochemistry Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Business & Management Journals

Ronald

[email protected]

1-702-714-7001Extn: 9042

Chemical Engineering and Chemistry Journals

Gabriel Shaw

[email protected]

1-702-714-7001 Extn: 9040

Earth & Environmental Sciences

Katie Wilson

[email protected]

1-702-714-7001Extn: 9042

Engineering Journals

James Franklin

[email protected]

1-702-714-7001Extn: 9042

General Science and Health care Journals

Andrea Jason

[email protected]

1-702-714-7001Extn: 9043

Genetics and Molecular Biology Journals

Anna Melissa

[email protected]

1-702-714-7001 Extn: 9006

Immunology & Microbiology Journals

David Gorantl

immuno[email protected]

1-702-714-7001Extn: 9014

Informatics Journals

Stephanie Skinner

[email protected]

1-702-714-7001Extn: 9039

Material Sciences Journals

Rachle Green

[email protected]

1-702-714-7001Extn: 9039

Mathematics and Physics Journals

Jim Willison

[email protected]

1-702-714-7001 Extn: 9042

Medical Journals

Nimmi Anna

[email protected]

1-702-714-7001 Extn: 9038

Neuroscience & Psychology Journals

Nathan T

[email protected]

1-702-714-7001Extn: 9041

Pharmaceutical Sciences Journals

John Behannon

[email protected]

1-702-714-7001Extn: 9007

Social & Political Science Journals

Steve Harry

[email protected]

1-702-714-7001 Extn: 9042

 
© 2008-2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version
adwords