alexa Interaction Of Nickel-dipeptide Complex With Ninhydrin In Aqueous And Micellar Media
ISSN: 2329-6798

Modern Chemistry & Applications
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International Conference on Applied Chemistry
October 17-18, 2016 Houston, USA

Neelam Hazoor Zaidi and Mohammad Akram
Fiji National University, Fiji
Aligarh Muslim University, India
Posters & Accepted Abstracts: Mod Chem appl
DOI: 10.4172/2329-6798.C1.003
Abstract
The kinetics of the title reaction have been investigated in both aqueous and micellar cetyltrimethylammonium bromide (CTAB) solutions. Ninhydrin reactions using manual and automated techniques as well as ninhydrin spray reagents are widely used to analyze and characterize amino acids, peptides, and proteins as well as numerous other ninhydrin positive compounds in biomedical, clinical, food, forensic, histochemical, microbiological, and nutritional and plant studies. Ninhydrin in aqueous solution reacts with α-amino acids to give a compound known as Ruhemann’s purple (diketohydrindylidenediketohydrindamine, DYDA). In order to elucidate the mechanism of the reactions of ninhydrin with [Ni(II)-Gly-Leu]+ complexes, systematic kinetic studies were performed by following appearance of the respective colored condensation products at different [surfactant], [reactant], [salt] and temperature. The rates of the reaction were determined in both water and surfactant micelles in the absence and presence of various organic and inorganic salts at 70 °C and pH 5.0 (acetic acid-sodium acetate buffer). The result shows that the reaction follows the same first-order and fractional-order kinetics with respect to [Ni (II)-Gly-Leu]+ and [ninhydrin], respectively, in both aqueous and micellar medium. Quantitative kinetic analysis of kψ−[CTAB] data were explained on the basis of the pseudo-phase model proposed by Menger and Portnoy and modified by Bunton and Rodenas.
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