alexa Intracellular Detection Of Infectious Pathogens In Sperm Cells
ISSN: 1948-5964

Journal of Antivirals & Antiretrovirals
Open Access

Like us on:
OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations

700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

Share This Page

Additional Info

Loading Please wait..

4th World Congress on Virology
October 06-08, 2014 Hilton San Antonio Airport, TX, USA

A D Gritzapis, K Makarounis, M Leventopoulos, E Nossi, D Nikolopoulos, G Georgoulias, V Kapetanios and V Tsilivakos
ScientificTracks Abstracts: J Antivir Antiretrovir
DOI: 10.4172/1948-5964.S1.020
Viral presence in sperm is a cause of infertility. Viral factors as a cause of male subfertility have not greatly concerned the medical community up until now. On the contrary, a correlation between high numbers of Natural Killer lymphocytes (ΝΚ) in the blood of women with a history of subfertility and/or miscarriages, and the presence of subclinical herpes viremia (HSV1-2, EBV, CMV, HHV6 and HHV7) has been described. Observation of miscarriage material revealed that NK mostly infiltrated at the implantation site, while the blood NK levels in a portion of these women were normal. This can be explained if the embryos in these cases were by themselves antigenic due to the presence of viral (at least herpes-viral) antigens, originating from the male through the sperm cells, including spermatozoa. These antigens would be expressed and presented to the woman's immune system by fetal cells causing the NK response. A method of intracellular detection of pathogens (viruses and Chlamydia trachomatis) has been developed. The sperm cells are fixed, permeabilized and DNA digestion is accomplished with DNase I. Incubation with antibody against each pathogen is followed by incubation with a fluorescent conjugated secondary antibody. The samples are acquired in a flow cytometry apparatus and analyzed with suitable software. Results so far, show that a significant percentage of samples taken from infertile men were found to be infected by intracellular chlamydia and/ or viruses. Especially in men infected with Chlamydia trachomatis, microbial load fell or the infection disappeared following antibiotic treatment. Furthermore, they also improve their Teratozoospermia Index (TZI) and the mean value of abnormalities per spermatozoon.
A. Gritzapis was granted a PhD at the age of 27 from the Department of Biological Chemistry in the Medical School of the University of Ioannina, Greece and was employed until 2012 as principal investigator at the Cancer Immunology Immunotherapy Center of the Department of Immunology at Saint Savas Cancer Hospital, Athens Greece. He is currently employed as principal investigator in the department of Immunology and Cellular Biology at Locus Medicus S.A., Athens, Greece. He has published more than 50 papers in reputed journals.
image PDF   |   image HTML
Peer Reviewed Journals
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

Agri, Food, Aqua and Veterinary Science Journals

Dr. Krish

1-702-714-7001 Extn: 9040

Clinical and Biochemistry Journals

Datta A

1-702-714-7001Extn: 9037

Business & Management Journals


1-702-714-7001Extn: 9042

Chemical Engineering and Chemistry Journals

Gabriel Shaw

1-702-714-7001 Extn: 9040

Earth & Environmental Sciences

Katie Wilson

1-702-714-7001Extn: 9042

Engineering Journals

James Franklin

1-702-714-7001Extn: 9042

General Science and Health care Journals

Andrea Jason

1-702-714-7001Extn: 9043

Genetics and Molecular Biology Journals

Anna Melissa

1-702-714-7001 Extn: 9006

Immunology & Microbiology Journals

David Gorantl

1-702-714-7001Extn: 9014

Informatics Journals

Stephanie Skinner

1-702-714-7001Extn: 9039

Material Sciences Journals

Rachle Green

1-702-714-7001Extn: 9039

Mathematics and Physics Journals

Jim Willison

1-702-714-7001 Extn: 9042

Medical Journals

Nimmi Anna

1-702-714-7001 Extn: 9038

Neuroscience & Psychology Journals

Nathan T

1-702-714-7001Extn: 9041

Pharmaceutical Sciences Journals

John Behannon

1-702-714-7001Extn: 9007

Social & Political Science Journals

Steve Harry

1-702-714-7001 Extn: 9042

© 2008-2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version