alexa Intraoperative Immunofluorescence Expresses Examination Of Effusionsfrom Serous Cavities
ISSN: 2157-7099

Journal of Cytology & Histology
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International Conference on Cytopathology
August 31-September 02, 2015 Toronto, Canada

Nadezhda Volchenko
Herzen Moscow Oncology Research Institute, Russia
Posters-Accepted Abstracts: J Cytol Histol
DOI: 10.4172/2157-7099.C1.003
The examination of effusions from serous cavities is the most complex part of cytological diagnostics. It is difficult to find cancer cells in effusions at early stages of a metastasis. Immunomorphological methods give real representation about a degree of prevalence of neoplastic process. Using immunocytochemical effusions examination by immunoperoxidase method (ICC) with usage of epithelial marker Ber-EP4, we showed that in complex diagnostic cases of adenogenic cancer in 79% of examinations, the dissemination of a tumor suspected by routine cytological examinations proves to be true in 15% not numerous tumor complexes which are not diagnosed at routine cytological research are revealed and in 7% it is possible to avoid overdiagnostics of neoplastic process. However, ICC examination is labor-intensive enough procedure and it occupies 3 hours on time. Immunofluorescence method (IF) with epithelial marker Ber-EP4 FITC is a new reliable and fast method of diagnostics of exudate character. Epithelial marker Ber-EP4 FITC (cloneBer-EP4) from “DAKO” company was directly carried in centrifugation deposit. Then liquid preparations were prepared with the help of Cytospin3 centrifuge. A staining by nuclear stain DAPI was carried out for visualization of nucleus of all cellular elements present in a preparation. Examination was carried out on fluorescence microscope Imager M1of “Karl Zeiss” company. Use of this method is especially perspective for intraoperative immunofluorescence express examination of effusions because it does not demand of complex preliminary preparation of material and substantial time expenses: An examination occupies 20 minutes. 600 examinations were carried out. Adenogenic cancer cells were found in 350 observations and in 65 ones tumor complexes were not numerous. At scheduled histological examinations in all 350 observations of specific effusions the dissemination of a tumor on serous sacs was confirmed. Test sensitivity was equal to 90% and specificity was 96%.

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