In-Vitro Cytotoxic Activity Of Methanolic Extract Of Hemidesmus Indicus R. Br. | 995
Journal of Cancer Science & Therapy
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R.Br.(Fam: Asclepidaceae) has been reported to be useful for the treatment of inflammation, cuts,
wounds, burns, snake bite, skin and blood diseases, ulcers, immunological disorders . In the present study, the methanolic
extract of the roots of
, was investigated against human colon cancer cell line (HT29) to explore its
anticancer potential. The effect of
methanolic extract (HIME) on proliferation of HT29 cancer cell line
was determined by microculture tetrazolium assay (MTT). The cells were exposed to different concentrations (100, 50 , 25, 12.5
, 6.25, 3.125 and1.5 μg/ml) of HIME or vehicle for 48 h. Cisplatin (5, 2.5 and 1.25 μg/ml) acted as positive control and vehicle
(DMSO) as negative control. Following treatment, the cells were exposed to Tetrazolium dye (5mg/ml) for 4 h. The formation
of the purple coloured formazan complex was dissolved by adding DMSO (100 μl) and read at 490 nm using ELISA microtiter
plate reader to determine the inhibitory concentration, IC50. About 40% increment in cell killing was seen when the dose of
HIME was increased from 1.5-25 μg/ml. At concentration of 100 μg/ml, 49.81%, cytotoxicity was recorded. The IC50 value of
HIME was 1.8964 μg/ml after 48 h of incubation. In this study, it was observed that HIME induces a concentration dependent
inhibition of HT29 cells, with an IC50 value of 1.8964 μg/ml after 48 h of incubation.
Mrs. Saumya Das is Persuing her Ph.D from Birla Institute of Technology, Ranchi, Mesra, India. She is a faculty of Pharmacology in Noida Institute
of Engineering and Technology, Greater Noida, India. She has published more than 12 papers in reputed International and National journals and
serving as an editorial board member and reviewer of repute. She is life member of Indian Pharmacological Society and Indian Pharmacy Graduate
Association. Her area of interest is Phytopharmacology, Toxicology and Anticancerous agents.
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