In-vitro protective effect of extracts of Premna integrifolia root against hydrogen peroxide induced oxidative damage

International Conference and Exhibition on Traditional & Alternative Medicine

December 09-11, 2013 Radisson Blu Plaza Hotel, Hyderabad, India

Prashant Y. Mali, Souravh S. Bais, Ajit R. Wankhede and Ashish Pandey

Posters: Altern Integ Med

Abstract :

The aim of present study was to evaluate in-vitro protective effect of extracts of P. integrifolia root on human leucocytes and erythrocytes against hydrogen peroxide induced oxidative damage. Chloroform: methanol (1:1) and aqueous extract of P. integrifolia roots were used to accessed catalase, superoxide dismutase, glutathione peroxidase, glutathione and lipid peroxidation levels in hydrogen peroxide induced oxidative damage. Results of these studies demonstrated that, there was an increase in the catalase, superoxide dismutase, glutathione peroxidase and reduction of the glutathione and lipid peroxidation levels in hydrogen peroxide group compared with control. P. integrifolia root extract treated groups showed that the reduction of catalase, superoxide dismutase, glutathione peroxidase and increased in the glutathione and lipid peroxidation levels as compared with hydrogen peroxide group. Chloroform: methanol (1:1) extract of P. integrifolia was found to be more effective than aqueous. It can be concluded that, the extracts of P. integrifolia root possessed in-vitro protective effect on human leucocytes and erythrocytes against hydrogen peroxide induced oxidative damage which has substantiated their use in ethnomedicine as an antioxidant. Observed effect attributed due to the flavonoid and phenol contents in the plant.