alexa
Reach Us +441474556909
Iron Oxide Nanoparticles Accelerate Apoptotic Death Of Human Neutrophils | 6591
ISSN: 2157-7439

Journal of Nanomedicine & Nanotechnology
Open Access

Like us on:

OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations
700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)
All submissions of the EM system will be redirected to Online Manuscript Submission System. Authors are requested to submit articles directly to Online Manuscript Submission System of respective journal.

Iron oxide nanoparticles accelerate apoptotic death of human neutrophils

International Conference and Exhibition on Nanotechnology & Nanomedicine

Diana Couto, Marisa Freitas, M. Arturo Lopez-Quintela, Jos? Rivas, Paulo Freitas, F?lix Carvalho and Eduarda Fernandes

Posters: J Nanomedic Nanotechnol

DOI: 10.4172/2157-7439.S1.07

Abstract
Nowadays, iron oxide nanoparticles have a great potential for being used in several areas of medicine, namely in drug delivery, cancer therapy and as contrast agents in imagiologic techniques, such as magnetic resonance imaging. However, the studies assessing the toxicity of these nanoparticles to the human health are scarce. Neutrophils are the first line of innate defense of the organism, and are essential in the defense of the organism against pro-inflammatory foreign bodies. Therefore, the aim of this study was to evaluate the effect of super paramagnetic iron oxide nanoparticles (magnetite form) on human neutrophils, namely its capacity to induce deleterious effects in these cells. For this purpose, necrotic cell death was measured by the trypan blue assay and lactate dehydrogenase leakage, apoptosis was evaluated by morphologic analysis, and the putative effect on mitochondrial membrane potential by using the probe 3,3?-dihexyloxacarbocyanine iodide. Finally, the modulation of the neutrophils? oxidative burst was studied using the probes dihydrorhodamine 123 and 2?,7?-dichlorofluorescein- diacetate. The obtained results suggest that these nanoparticles have the ability to accelerate the neutrophils? apoptotic process. Thus, these results could have important implications in the innate defense of the organism, during the above mentioned application of iron oxide nanoparticles for medical purposes.
Biography
Diana Couto, PharmD, is a PhD student at the Faculty of Pharmacy, University of Porto, Portugal
Top